Bone‐marrow mesenchymal stem cell transplantation to treat diabetic nephropathy in tree shrews. (28th May 2014)

• Record Type: Journal Article
• Authors: Pan, Xing‐Hua; Yang, Xiao‐Yan; Yao, Xiang; Sun, Xiao‐Mei; Zhu, Lu; Wang, Jin‐Xiang; Pang, Rong‐Qing; Cai, Xue‐Min; Dai, Jie‐Jie; Ruan, Guang‐Ping
• Is Part Of: Cell biochemistry and function. Volume 32:Number 5(2013:Jul.)
• Page Start: 453
• Page End: 463
• Publication Date: 2014-05-28
• Subjects: Cytochemistry -- Periodicals; Cell metabolism -- Periodicals; Biochemistry -- Periodicals; Cytology -- Periodicals; 572
• DOI: 10.1002/cbf.3037
• Languages: English
• ISSNs: 0263-6484

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Diabetic nephropathy (DN) is a common microvascular complication of diabetes. We used a new DN model in tree shrews to validate the use of bone‐marrow mesenchymal stem cell (BM‐MSC) transplantation to treat DN. The DN tree shrew model was established by a high‐sugar and high‐fat diet and four injections of streptozotocin. 4', 6‐Diamidino‐2‐phenylindole labelled BM‐MSCs were injected into tree shrews. The DN tree shrew model was successfully established. Blood glucose was significantly increased ( <italic>p</italic> &lt; 0.01) during the entire experiment. DN tree shrews showed dyslipidemia, insulin resistance and increased 24‐h proteinuria. At 21 days after BM‐MSC transplantation, glucose and levels of triglycerides, total cholesterol and 24‐h urine volume were lower than in tree shrews with DN alone ( <italic>p</italic> &lt; 0.01) but were still higher than control values ( <italic>p</italic> &lt; 0.01). Levels of creatinine and urea nitrogen as well as 24‐h proteinuria were lower for DN tree shrews with BM‐MSCs transplantation than DN alone ( <italic>p</italic> &lt; 0.05). High‐sugar and high‐fat diet combined with STZ injection can induce a tree shrew model of DN. BM‐MSCs injection can home to damaged kidneys and pancreas, for reduced 24‐h proteinuria and improved insulin resistance. Copyright © 2014 John Wiley &amp; Sons, Ltd.</p> </abstract>