Analysis and comparison of oxygen consumption of HepG2 cells in a monolayer and three‐dimensional high density cell culture by use of a matrigrid®. Issue 9 (22nd April 2013)

• Record Type: Journal Article
• Authors: Weise, Frank; Fernekorn, Uta; Hampl, Jörg; Klett, Maren; Schober, Andreas
• Is Part Of: Biotechnology and bioengineering. Volume 110:Issue 9(2013:Sep.)
• Page Start: 2504
• Page End: 2512
• Publication Date: 2013-04-22
• Subjects: Biotechnology -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/bit.24912
• Languages: English
• ISSNs: 0006-3592

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>ABSTRACT</title> <sec id="bit24912-sec-0001" sec-type="section"> <p>By the use of a MatriGrid® we have established a three‐dimensional high density cell culture. The MatriGrid® is a culture medium permeable, polymeric scaffold with 187 microcavities. In these cavities (300 μm diameter and 207 μm deep) the cells can growth three‐dimensionally. For these experiments we measured the oxygen consumption of HepG2 cell cultures in order to optimize cultivation conditions. We measured and compared the oxygen consumption, growth rate and vitality under three different cultivation conditions: monolayer, three‐dimensional static and three‐dimensional actively perfused. The results show that the cells in a three‐dimensional cell culture consume less oxygen as in a monolayer cell culture and that the actively perfused three‐dimensional cell culture in the MatriGrid® has a similar growth rate and vitality as the monolayer culture. Biotechnol. Bioeng. 2013; 110:2504–2512. © 2013 Wiley Periodicals, Inc.</p> </sec> </abstract>