A multi‐dimensional approach for fractionating proteins using charged membranes. (9th February 2013)

• Record Type: Journal Article
• Authors: Sorci, Mirco; Gu, Minghao; Heldt, Caryn L.; Grafeld, Elizabeth; Belfort, Georges
• Is Part Of: Biotechnology and bioengineering. Volume 110:Number 6(2013:Jun.)
• Page Start: 1704
• Page End: 1713
• Publication Date: 2013-02-09
• Subjects: Biotechnology -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/bit.24837
• Languages: English
• ISSNs: 0006-3592

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>In an effort to increase selectivity among proteins with crossflow ultrafiltration, we offer and demonstrate a comprehensive approach to fractionate proteins of similar molecular weight and relatively close p<italic>I</italic> values. This multidimensional approach involves optimizing membrane charge type and density together with operating conditions such as precise control of pH, ionic strength, and transmembrane pressure for reduced membrane fouling. Each filtration experiment was performed in cross‐flow configuration for ∼20 min, allowing fast screening for optimal separation as determined by maximum selectivity, <italic>Ψ</italic>, and purity, <italic>P</italic>. Using our comprehensive approach for fractionating mixtures RNase A–lysozyme and BSA–hemoglobin, we obtained values of <italic>Ψ</italic> = 9.1, <italic>P</italic> = 95.7%, and <italic>Ψ</italic> = 6.5, <italic>P</italic> = 62.1%, respectively. Biotechnol. Bioeng. 2013; 110: 1704–1713. © 2013 Wiley Periodicals, Inc.</p> </abstract>