Blocking of the ATP sensitive potassium channel ameliorates the ischaemia‐reperfusion injury in the rat testis. (7th March 2014)

• Record Type: Journal Article
• Authors: Shimizu, S.; Oikawa, R.; Tsounapi, P.; Inoue, K.; Shimizu, T.; Tanaka, K.; Martin, D. T.; Honda, M.; Sejima, T.; Tomita, S.; Saito, M.
• Is Part Of: Andrology. Volume 2:Number 3(2014)
• Page Start: 458
• Page End: 465
• Publication Date: 2014-03-07
• Subjects: Andrology -- Periodicals; 616.65
• DOI: 10.1111/j.2047-2927.2014.00199.x
• Languages: English
• ISSNs: 2047-2919

Abstract

<abstract abstract-type="main" id="andr199-abs-0001"> <title>Summary</title> <p>There is increasing evidence that the effects of administered ATP sensitive potassium (K<sub>ATP</sub>) channel openers or blockers during ischaemia are still controversial in many organs/tissues. Testicular torsion detorsion which causes ischaemia‐reperfusion (IR) injury, cannot be predicted, thus an effective drug should be administered during or after the ischaemia. The aim of this study was to examine whether the administration of K<sub>ATP</sub> channel openers or blockers during ischaemia ameliorates IR injury in the testis. Eight‐week‐old male Sprague–Dawley rats were subjected to 2 h right testicular ischaemia followed by 24 h reperfusion. The selective mitochondrial (mito) K<sub>ATP</sub> channel blocker, 5‐hydroxydecanoate (5‐HD) (40 mg/kg), the non‐selective K<sub>ATP</sub> channel blocker glibenclamide (5 mg/kg), the selective mito K<sub>ATP</sub> channel opener diazoxide (10 mg/kg) and the non‐selective K<sub>ATP</sub> channel opener cromakalim (300 μg/kg) were administered intraperitoneally 15 min prior to the ischaemia or 75 min after the induction of ischaemia. Tissue damage was evaluated by malondialdehyde concentration, myeloperoxidase activity, histological evaluation and TdT‐mediated dUTP nick end labelling assay in the testis. There was a significant increase in oxidative stress, neutrophil infiltration, histological damage and apoptosis in the testicular IR model. A significant reduction in the testicular IR injury was observed with the administration of glibenclamide, but not 5‐HD, diazoxide or cromakalim during ischaemia. The administration of non‐selective K<sub>ATP</sub> channel blocker glibenclamide ameliorated the testicular IR injury. On the other hand, the selective mito K<sub>ATP</sub> channel blocker, 5‐HD and K<sub>ATP</sub> channel openers did not reduce the testicular IR injury. These data suggest that blocking of the membrane K<sub>ATP</sub> channel may have a protective effect during the testicular ischaemia. Glibenclamide could be an effective drug to manage the post‐ischaemic injury caused by the testicular torsion‐detorsion.</p> </abstract>