Atg21 regulates pexophagy via its PI(3)P‐binding activity in Pichia pastoris. Issue 3 (23rd January 2014)

• Record Type: Journal Article
• Authors: Tamura, Naoki; Oku, Masahide; Sakai, Yasuyoshi
• Is Part Of: FEMS yeast research. Volume 14:Issue 3(2014)
• Page Start: 435
• Page End: 444
• Publication Date: 2014-01-23
• Subjects: Yeast -- Periodicals; Yeasts -- Periodicals; 579.562
• DOI: 10.1111/1567-1364.12132
• Languages: English
• ISSNs: 1567-1356

Abstract

<abstract abstract-type="main" id="fyr12132-abs-0001"> <title>Abstract</title> <p>Pexophagy is a selective degradation pathway of peroxisomes. In the present study, we revealed that PpAtg21 was required for pexophagy in the methylotrophic yeast <italic>Pichia pastoris</italic>. PpAtg21 was essential for efficient lipidation of Atg8 and for <italic>de novo</italic> synthesis of pexophagic membranes. In contrast to PpAtg18, PpAtg21 was not necessary for vacuolar fission nor invagination during micropexophagy. PpAtg21 specifically bound to PI(3)P, but not to PI(3, 5)P₂<italic>in vitro</italic>, and the localization analyses matched with this phosphoinositide‐binding specificity. The mutant which lost the lipid‐binding activity showed defect in pexophagy, suggesting that PI(3)P‐binding activity of PpAtg21 was required for pexophagy.</p> </abstract>