Influence of bone marrow‐derived mesenchymal stem cells pre‐implantation differentiation approach on periodontal regeneration in vivo. (12th March 2015)

• Record Type: Journal Article
• Authors: Cai, Xinjie; Yang, Fang; Yan, Xiangzhen; Yang, Wanxun; Yu, Na; Oortgiesen, Daniel A. W.; Wang, Yining; Jansen, John A.; Walboomers, X. Frank
• Is Part Of: Journal of clinical periodontology. Volume 42:Number 4(2015:Apr.)
• Page Start: 380
• Page End: 389
• Publication Date: 2015-03-12
• Subjects: Periodontics -- Periodicals; 617.6
• DOI: 10.1111/jcpe.12379
• Languages: English
• ISSNs: 0303-6979

Abstract

<abstract abstract-type="main" id="jcpe12379-abs-0001"> <title>Abstract</title> <sec id="jcpe12379-sec-0001" sec-type="section"> <title>Aim</title> <p>The implantation of bone marrow‐derived mesenchymal stem cells (MSCs) has previously been shown successful to achieve periodontal regeneration. However, the preferred pre‐implantation differentiation strategy (e.g. maintenance of stemness, osteogenic or chondrogenic induction) to obtain optimal periodontal regeneration is still unknown. This in vivo study explored which differentiation approach is most suitable for periodontal regeneration.</p> </sec> <sec id="jcpe12379-sec-0002" sec-type="section"> <title>Materials and Methods</title> <p>Mesenchymal stem cells were obtained from Fischer rats and seeded onto poly(lactic‐co‐glycolic acid)/poly(<italic>ɛ</italic>‐caprolactone) electrospun scaffolds, and then pre‐cultured under different in vitro conditions: (i) retention of multilineage differentiation potential; (ii) osteogenic differentiation approach; and (iii) chondrogenic differentiation approach. Subsequently, the cell‐scaffold constructs were implanted into experimental periodontal defects of Fischer rats, with empty scaffolds as controls. After 6 weeks of implantation, histomorphometrical analyses were applied to evaluate the regenerated periodontal tissues.</p> </sec> <sec id="jcpe12379-sec-0003" sec-type="section"> <title>Results</title> <p>The chondrogenic differentiation approach showed regeneration of alveolar bone and ligament tissues. The retention of multilineage differentiation potential supported only ligament regeneration, while the osteogenic differentiation approach boosted alveolar bone regeneration.</p> </sec> <sec id="jcpe12379-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Chondrogenic differentiation of MSCs before implantation is a useful strategy for regeneration of alveolar bone and periodontal ligament, in the currently used rat model.</p> </sec> </abstract>