Inflorescences vs leaves: a distinct modulation of carbon metabolism process during Botrytis infection. Issue 1 (6th November 2014)

• Record Type: Journal Article
• Authors: Vatsa‐Portugal, Parul; Walker, Anne‐Sophie; Jacquens, Lucile; Clément, Christophe; Barka, Essaid Ait; Vaillant‐Gaveau, Nathalie
• Is Part Of: Physiologia plantarum. Volume 154:Issue 1(2015:May)
• Page Start: 162
• Page End: 177
• Publication Date: 2014-11-06
• Subjects: Plant physiology -- Periodicals; 571.2
• DOI: 10.1111/ppl.12287
• Languages: English
• ISSNs: 0031-9317

Abstract

<abstract abstract-type="main" id="ppl12287-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p id="ppl12287-para-0001">Plant growth and survival depends critically on photo assimilates. Pathogen infection leads to changes in carbohydrate metabolism of plants. In this study, we monitored changes in the carbohydrate metabolism in the grapevine inflorescence and leaves using <italic>Botrytis cinerea</italic> and <italic>Botrytis pseudo cinerea</italic>. Fluctuations in gas exchange were correlated with variations in chlorophyll <italic>a</italic> fluorescence. During infection, the inflorescences showed an increase in net photosynthesis (Pn) with a stomatal limitation. In leaves, photosynthesis decreased, with a non‐stomatal limitation. A decrease in the effective photosystem II (PSII) quantum yield (ΦPSII) was accompanied by an increase in photochemical quenching (qP) and non‐photochemical quenching (qN). The enhancement of qP and ΦPSII could explain the observed increase in Pn. In leaves, the significant decline in ΦPSII and qP, and increase in qN suggest that energy was mostly oriented toward heat dissipation instead of CO<sub>2</sub> fixation. The accumulation of glucose and sucrose in inflorescences and glucose and fructose in the leaves during infection indicate that the plant's carbon metabolism is differently regulated in these two organs. While a strong accumulation of starch was observed at 24 and 48 hours post‐inoculation (hpi) with both species of <italic>Botrytis</italic> in the inflorescences, a significant decrease with <italic>B. cinerea</italic> at 24 hpi and a significant increase with <italic>B. pseudo cinerea</italic> at 48 hpi were observed in the leaves. On the basis of these results, it can be said that during pathogen attack, the metabolism of grapevine inflorescence and leaf is modified suggesting distinct mechanisms modifying gas exchange, PSII activity and sugar contents in these two organs.</p> </abstract>