TTAGG‐repeat telomeres and characterization of telomerase in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae). Issue 3 (17th February 2015)

• Record Type: Journal Article
• Authors: Gong, H.; Zhu, W.; Zhang, J.; Li, X.; Meng, Q.; Zhou, G.; Wang, M.; Wang, H.; Miao, L.; Qin, Q.; Zhang, H.
• Is Part Of: Insect molecular biology. Volume 24:Issue 3(2015:Jun.)
• Page Start: 358
• Page End: 367
• Publication Date: 2015-02-17
• Subjects: Insects -- Molecular aspects -- Periodicals; 595.7
• DOI: 10.1111/imb.12163
• Languages: English
• ISSNs: 0962-1075

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Telomeres are maintained usually by telomerase, a specialized reverse transcriptase that adds this sequence to chromosome ends. In this study, telomerase activity was detected in the in different somatic tissues, such as midgut and fat bodies, by the telomeric repeat amplification protocol (TRAP) in <italic>Spodoptera exigua</italic>. The structure of the telomeres of <italic>S. exigua</italic> was evaluated by sequence analysis of the TRAP products, revealing that the telomerase synthesized a (TTAGG)n repeat. The presence of a telomerase reverse transcriptase (TERT) subunit coding gene has been cloned, sequenced and expressed in vitro successively. Notably, the <italic>S. exigua</italic> telomerase (SpexTERT) gene structure lacks the N‐terminal GQ motif. Telomerase contains a large RNA subunit, TER, and a protein catalytic subunit, TERT. Here we report an in vitro system that was reconstructed by all components of the telomerase complex, a purified recombinant SpexTERT without a N‐terminal GQ motif and a mutant human telomerase RNA (TER), showed telomerase activity. Together, these results suggest the GQ motif is not essential for telomerase catalysis.</p> </abstract>