Active Targeting of Tumors through Conformational Epitope Imprinting1. Issue 17 (26th February 2015)

• Record Type: Journal Article
• Authors: Zhang, Yan; Deng, Chunyue; Liu, Sha; Wu, Jin; Chen, Zhangbao; Li, Chong; Lu, Weiyue
• Is Part Of: Angewandte Chemie international edition. Volume 54:Issue 17(2015)
• Page Start: 5157
• Page End: 5160
• Publication Date: 2015-02-26
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201412114
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Inspired by the knowledge that most antibodies recognize a conformational epitope because of the epitope's specific three‐dimensional shape rather than its linear structure, we combined scaffold‐based peptide design and surface molecular imprinting to fabricate a novel nanocarrier harboring stable binding sites that captures a membrane protein. In this study, a disulfide‐linked α‐helix‐containing peptide, apamin, was used to mimic the extracellular, structured N‐terminal part of the protein p32 and then serve as an imprinting template for generating a sub‐40 nm‐sized polymeric nanoparticle that potently binds to the target protein, recognizes p32‐positive tumor cells, and successfully mediates targeted photodynamic therapy in vivo. This could provide a promising alternative for currently used peptide‐modified nanocarriers and may have a broad impact on the development of polymeric nanoparticle‐based therapies for a wide range of human diseases.</p> </abstract>