Insulin‐independent actions of glucagon‐like peptide‐1 in wethers. (30th November 2014)

• Record Type: Journal Article
• Authors: El‐Sabagh, Mabrouk; Taniguchi, Dai; Sugino, Toshihisa; Obitsu, Taketo; Taniguchi, Kohzo
• Is Part Of: Animal science journal. Volume 86:Number 4(2015:Apr.)
• Page Start: 385
• Page End: 391
• Publication Date: 2014-11-30
• Subjects: Animal culture -- Periodicals; Livestock -- Periodicals; 636
• DOI: 10.1111/asj.12306
• Languages: English
• ISSNs: 1344-3941

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Insulin‐independent actions of glucagon‐like peptide‐1 (GLP‐1) are not yet clear in ruminants. Four Suffolk mature wethers (60.0 ± 6.7 kg body weight (BW)) were intravenously infused with insulin (0.5 mU/kg BW/min; from 0 to 90 min) and GLP‐1 (0.5 μg/kg BW/min; from 60 to 150 min) with both hormones co‐administered from 60 to 90 min, in a repeated‐measure design under euglycemic clamp for 150 min, to investigate whether GLP‐1 has insulin‐independent actions. Jugular blood samples were taken at 15‐min intervals for plasma hormones and metabolites analysis. Compared to baseline concentrations (at 0 min), insulin infusion decreased (<italic>P</italic> < 0.05) plasma concentrations of glucagon, non‐esterified fatty acids (NEFA), lactate, nonessential amino acids (NEAA), branched‐chain amino acids (BCAA), total amino acids (TAA) and urea nitrogen (UN). Insulin plus GLP‐1 infusion induced a greater increase (<italic>P</italic> < 0.05) in plasma concentrations of insulin and triglyceride (TG), but decreased (<italic>P</italic> < 0.05) glucagon, total cholesterol (T‐Cho), NEAA and UN plasma concentrations. GLP‐1 infusion increased (<italic>P</italic> < 0.05) NEFA, β‐hydroxybutyrate and TG, but decreased (<italic>P</italic> < 0.05) glucagon, T‐Cho, NEAA, BCAA and UN plasma concentrations. In conclusion, GLP‐1 exerts extrapancreatic roles in ruminants not only insulin‐independent but probably, in contrast to non‐ruminants, antagonistic to insulin effects.</p> </abstract>