Comparison of Platelet Counting Technologies in Equine Platelet Concentrates. Issue 3 (13th October 2014)

• Record Type: Journal Article
• Authors: O'Shea, Caitlin M.; Werre, Stephen R.; Dahlgren, Linda A.
• Is Part Of: Veterinary surgery. Volume 44:Issue 3(2015)
• Page Start: 304
• Page End: 313
• Publication Date: 2014-10-13
• Subjects: Veterinary surgery -- Periodicals; Veterinary Medicine -- Periodicals; Surgery -- Periodicals; Societies, Medical -- Periodicals; 636.0897
• DOI: 10.1111/j.1532-950X.2014.12290.x
• Languages: English
• ISSNs: 0161-3499

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="vsu12290-sec-0001" sec-type="section"> <title>Objective</title> <p>(1) To compare the performance of 4 platelet counting technologies in equine platelet concentrates and (2) to evaluate the ability of the Magellan platelet rich plasma (PRP) system to concentrate equine platelets.</p> </sec> <sec id="vsu12290-sec-0002" sec-type="section"> <title>Study Design</title> <p>Experimental study to assess method agreement.</p> </sec> <sec id="vsu12290-sec-0003" sec-type="section"> <title>Animals</title> <p>Adult mixed breed horses (n = 32).</p> </sec> <sec id="vsu12290-sec-0004" sec-type="section"> <title>Methods</title> <p>Acid citrate dextrose‐A anti‐coagulated whole blood was collected and PRP produced using the Magellan system according to the manufacturer's instructions. Platelets were quantified using 4 counting methods: optical scatter (Advia 2120), impedance (CellDyn 3700), hand counting, and fluorescent antibody flow cytometry. Platelet concentrations were compared using Passing and Bablok regression analyses and mixed model ANOVA. Significance was set at <italic>P</italic> < .05.</p> </sec> <sec id="vsu12290-sec-0005" sec-type="section"> <title>Results</title> <p>Platelet concentrations measured in identical PRP samples were consistently higher for the Advia 2120 than the CellDyn 3700. Systematic and proportional biases were observed between these 2 automated methods when analyzed by regression analysis of the larger sample size. No bias (systematic or proportional) was observed among any of the other counting methods. Despite the bias detected between the 2 automated systems, there were no significant differences on average among the 4 counting methods evaluated, based on the ANOVA. The Magellan system consistently generated high platelet concentrations as well as higher than expected WBC concentrations.</p> </sec> <sec id="vsu12290-sec-0006" sec-type="section"> <title>Conclusions</title> <p>The Magellan system delivered desirably high platelet concentrations; however, WBC concentrations may be unacceptably high for some orthopedic applications. All 4 platelet counting methods tested were equivalent on average and therefore suitable for quantifying platelets in equine PRP used for clinical applications.</p> </sec> </abstract>