Systematic Separation and Purification of Iridoid Glycosides and Crocetin Derivatives from Gardenia jasminoides Ellis by High‐speed Counter‐current Chromatography. Issue 3 (27th January 2015)

• Record Type: Journal Article
• Authors: Wang, Yarong; Chen, Yang; Deng, Liang; Cai, Shining; Liu, Jia; Li, Wenna; Du, Lianfeng; Cui, Guozhen; Xu, Xin; Lu, Tao; Chen, Ping; Zhang, Hao
• Is Part Of: Phytochemical analysis. Volume 26:Issue 3(2015:May)
• Page Start: 202
• Page End: 208
• Publication Date: 2015-01-27
• Subjects: Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2553
• Languages: English
• ISSNs: 0958-0344

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="pca2553-sec-0001" sec-type="section"> <title>Introduction</title> <p>Iridoid glycosides and crocetin derivatives are the main bioactive components of <italic>Gardenia</italic>. The processes of separation of these compounds reported in much of the literature are tedious, time consuming and require multiple chromatographic steps, which results in lower recovery and higher costs.</p> </sec> <sec id="pca2553-sec-0002" sec-type="section"> <title>Objective</title> <p>To develop a high‐speed counter‐current chromatography (HSCCC) method for the systematic separation and purification of iridoid glycosides and crocetin derivatives on a preparative scale from <italic>Gardenia</italic>.</p> </sec> <sec id="pca2553-sec-0003" sec-type="section"> <title>Methods</title> <p>After fractionation using HPD100 column chromatography, <italic>n</italic>‐butanol:ethanol:water (10:1:10, v/v) was selected to purify gardenoside, 6<italic>β</italic>‐hydroxy geniposide and geniposidic acid from fraction A; ethyl acetate:<italic>n</italic>‐butanol:water (2:1.5:3, v/v) was used to isolate geniposide from fraction B; crocin‐1, crocin‐2, crocin‐3 and crocin‐4 were purified by hexane:ethyl acetate:<italic>n</italic>‐butanol:water (1:2:1:5, v/v) from fraction C. The head‐to‐tail elution mode was used with a flow rate of 8.0 mL/min and a rotary speed of 600 rpm.</p> </sec> <sec id="pca2553-sec-0004" sec-type="section"> <title>Results</title> <p>After HSCCC isolation, 151.1 mg of gardenoside, 52.2 mg of 6<italic>β</italic>‐hydroxy geniposide and 24.5 mg of geniposidic acid were obtained from 800 mg of fraction A; 587.2 mg of geniposide was obtained from 800 mg of Fraction B; 246.2 mg of crocin‐1, 34.2 mg of crocin‐2, 24.4 mg of crocin‐3 and 24.7 mg of crocin‐4 were obtained from 1000 mg of fraction C. Their purities were found by UPLC analysis to be 91.7%, 93.4%, 92.5%, 98.2%, 94.1%, 96.3%, 94.1% and 98.9% respectively.</p> </sec> <sec id="pca2553-sec-0005" sec-type="section"> <title>Conclusion</title> <p>The present results demonstrates that the main iridoid glycosides and crocetin derivatives in <italic>Gardenia</italic> can be obtained efficiently from extracts using HSCCC. Copyright © 2015 John Wiley & Sons, Ltd.</p> </sec> </abstract>