A molecular diagnostic assay for the detection and identification of wood decay fungi of conifers. Issue 2 (15th July 2014)

• Record Type: Journal Article
• Authors: Gonthier, P.; Guglielmo, F.; Sillo, F.; Giordano, L.; Garbelotto, M.; Lakomy, P.
• Is Part Of: Forest pathology. Volume 45:Issue 2(2015:Apr.)
• Page Start: 89
• Page End: 101
• Publication Date: 2014-07-15
• Subjects: Trees -- Diseases and pests -- Periodicals; Trees -- Effect of air pollution on -- Periodicals; Forests and forestry -- Environmental aspects -- Periodicals; 634.9
• DOI: 10.1111/efp.12132
• Languages: English
• ISSNs: 1437-4781

Abstract

<abstract abstract-type="main" id="efp12132-abs-0001"> <title>Summary</title> <p>Ten taxon‐specific primers were designed to amplify the Internal Transcribed Spacer of the rRNA operon of several important decay fungi of coniferous wood, including <italic>Armillaria</italic> spp., <italic>Echinodontium</italic> spp., <italic>Fomitopsis pinicola</italic>, <italic> Fuscoporia torulosa</italic>, <italic> Heterobasidion annosum sensu lato</italic> (<italic>s.l</italic>.), <italic>Onnia</italic> spp., <italic>Phaeolus schweinitzii</italic>, <italic> Phellinus weirii s.l</italic>., <italic>Pholiota</italic> spp. and <italic>Porodaedalea</italic> spp. Primers designed in this study and in a previous one for the identification of <italic>Laetiporus sulphureus</italic> and <italic>Stereum</italic> spp. were combined in two multiplex PCRs, which were tested for efficiency and specificity, and detected at least 1 pg of fungal target DNA. Target DNA at concentrations of 10⁻¹ pg or lower can be detected with this assay using SYBR^® Green Real‐Time PCR. Validation assays performed on 129 naturally infected wood samples or fruiting bodies confirmed the reliability of the multiplex PCR‐based diagnostic method. This method represents a simple and rapid diagnostic tool for the detection of a number of destructive wood decay fungi of conifer wood.</p> </abstract>