Bile‐induced peptidoglycan remodelling in Salmonella enterica. (24th June 2014)

• Record Type: Journal Article
• Authors: Hernández, Sara B.; Cava, Felipe; Pucciarelli, M. Graciela; García‐del Portillo, Francisco; de Pedro, Miguel A.; Casadesús, Josep
• Is Part Of: Environmental microbiology. Volume 17:Number 4(2015:Apr.)
• Page Start: 1081
• Page End: 1089
• Publication Date: 2014-06-24
• Subjects: Microbial ecology -- Periodicals; Environmental Microbiology -- Periodicals; 579.17
• DOI: 10.1111/1462-2920.12491
• Languages: English
• ISSNs: 1462-2912

Abstract

<abstract abstract-type="main"> <title>Summary</title> <p>Changes in the peptidoglycan (PG) structure of <italic>S</italic><italic>almonella enterica</italic> are detected in the presence of a sublethal concentration of sodium deoxycholate (DOC): (i) lower proportions of Braun lipoprotein (Lpp)‐bound muropeptides; (ii) reduced levels of muropeptides cross‐linked by L(meso)‐diaminopimelyl‐D(meso)‐diaminopimelic acid (L‐D) peptide bridges (3‐3 cross‐links). Similar structural changes are found in <italic>S</italic><italic>. enterica</italic> cultures adapted to grow in the presence of a lethal concentration of DOC, suggesting that reduced anchoring of Braun protein to PG and low occurrence of 3‐3 cross‐links may increase <italic>S</italic><italic>. enterica</italic> resistance to bile. This view is further supported by additional observations: (i) A triple mutant lacking L, D‐transpeptidases YbiS, ErfK, and YcfS, which does not contain Lpp anchored to PG, is hyper‐resistant to bile; (ii) enhanced 3‐3 cross‐linking upon overexpression of YnhG transpeptidase causes a decrease in bile resistance. These observations suggest that remodelling of the cell wall may be added to the list of adaptive responses that permit survival of <italic>S</italic><italic>. enterica</italic> in the presence of bile.</p> </abstract>