Effect of short‐term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry. (9th April 2014)

• Record Type: Journal Article
• Authors: Trigo, P.; Merino, O.; Figueroa, E.; Valdebenito, I.; Sánchez, R.; Risopatrón, J.
• Is Part Of: Andrologia. Volume 47:Number 4(2015)
• Page Start: 407
• Page End: 411
• Publication Date: 2014-04-09
• Subjects: Andrology -- Periodicals; Infertility, Male -- Periodicals; 616.69
• DOI: 10.1111/and.12276
• Languages: English
• ISSNs: 0303-4569

Abstract

<abstract abstract-type="main" id="and12276-abs-0001"> <title>Summary</title> <p>The short‐term storage of salmonid semen is a viable method for <italic>in vitro</italic> fertilisation. Previous studies have found that short‐term storage affects sperm motility, compromising quality and fertilising capacity. However, the functional characteristics of the spermatozoa of <italic>O. mykiss</italic> during storage time and its relation to the spawning period are little known. This study was designed to evaluate the effects of <italic>in vitro</italic> short‐term storage on sperm functional parameters in <italic>O. mykiss, </italic> determined by flow cytometry. Semen samples of the first spawning – undiluted (SSD) and diluted (SD) (Storfish<sup>®</sup> 1 : 2v/v; IMV AI solutions, France) – were stored at 4 °C for 14 days. Motility, viability (PMI: plasma membrane integrity) and mitochondrial membrane potential (ΔΨM) were assessed. On the fifth day of storage, spermatozoa showed a motility >70% (SSD: 78.3% versus SD 85.0%), PMI (81.5% SSD/87.2% SD) and ΔΨM (72.5% SSD/SD 80.0%) (<italic>P</italic> < 0.05). However, a significant decline in the percentage of all functional parameters (<italic>P</italic> < 0.05) was observed after 5 days of storage for all samples of both undiluted (SSD) and diluted semen. In conclusion, the results here provide new data on <italic>O. mykiss</italic> sperm quality with respect to <italic>in vitro</italic> short‐term storage evaluated by flow cytometry.</p> </abstract>