The novel strain Fusarium proliferatum LE1 (RCAM02409) produces α‐L‐fucosidase and arylsulfatase during the growth on fucoidan. (24th October 2014)

• Record Type: Journal Article
• Authors: Shvetsova, Svetlana V.; Zhurishkina, Elena V.; Bobrov, Kirill S.; Ronzhina, Natalia L.; Lapina, Irina M.; Ivanen, Dina R.; Gagkaeva, Tatiana Yu.; Kulminskaya, Anna A.
• Is Part Of: Journal of basic microbiology. Volume 55:issue 4(2015:Apr.)
• Page Start: 471
• Page End: 479
• Publication Date: 2014-10-24
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.201400309
• Languages: English
• ISSNs: 0233-111X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jobm201400309-sec-0001" sec-type="section"> <p>Enzymes capable of modifying the sulfated polymeric molecule of fucoidan are mainly produced by different groups of marine organisms: invertebrates, bacteria, and also some fungi. We have discovered and identified a new strain of filamentous fungus <italic>Fusarium proliferatum</italic> LE1 (deposition number in Russian Collection of Agricultural Microorganisms is RCAM02409), which is a potential producer of fucoidan‐degrading enzymes. The strain LE1 (RCAM02409) was identified on the basis of morphological characteristics and analysis of ITS sequences of ribosomal DNA. During submerged cultivation of <italic>F. proliferatum</italic> LE1 in the nutrient medium containing natural fucoidan sources (the mixture of brown algae <italic>Laminaria digitata</italic> and <italic>Fucus vesiculosus</italic>), enzymic activities of α‐<sc>L</sc>‐fucosidase and arylsulfatase were inducible. These enzymes hydrolyzed model substrates, <italic>para</italic>‐nitrophenyl α‐<sc>L</sc>‐fucopyranoside and <italic>para</italic>‐nitrophenyl sulfate, respectively. However, the α‐<sc>L</sc>‐fucosidase is appeared to be a secreted enzyme while the arylsulfatase was an intracellular one. No detectable fucoidanase activity was found during <italic>F. proliferatum</italic> LE1 growth in submerged culture or in a static one. Comparative screening for fucoidanase/arylsulfatase/α‐<sc>L</sc>‐fucosidase activities among several related <italic>Fusarium</italic> strains showed a uniqueness of <italic>F. proliferatum</italic> LE1 to produce arylsulfatase and α‐<sc>L</sc>‐fucosidase enzymes. Apart them, the strain was shown to produce other glycoside hydrolyses.</p> </sec> </abstract>