Co‐Transplantation of Endothelial Progenitor Cells and Mesenchymal Stem Cells Promote Neovascularization and Bone Regeneration. (12th July 2013)

• Record Type: Journal Article
• Authors: Zigdon‐Giladi, Hadar; Bick, Tova; Lewinson, Dina; Machtei, Eli E.
• Is Part Of: Clinical implant dentistry and related research. Volume 17:Number 2(2015:Apr.)
• Page Start: 353
• Page End: 359
• Publication Date: 2013-07-12
• Subjects: Dental implants -- Periodicals; Dental Implantation -- Periodicals; Dental Implants -- Periodicals; 617.693
• DOI: 10.1111/cid.12104
• Languages: English
• ISSNs: 1523-0899

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="cid12104-sec-0001" sec-type="section"> <title>Background</title> <p>Bone formation relies on sufficient blood supply and osteoprogenitor cells.</p> </sec> <sec id="cid12104-sec-0002" sec-type="section"> <title>Purpose</title> <p>The study aims to evaluate the influence of endothelial progenitor cells (EPCs) in combination with mesenchymal stem cells (MSCs) on early vascularization and intramembranous bone regeneration.</p> </sec> <sec id="cid12104-sec-0003" sec-type="section"> <title>Materials and Methods</title> <p>Vertical bone regeneration was tested in rat calvarium guided bone regeneration model. Gold domes were filled with a mixture of 5 × 10<sup>5</sup> osteogenic transformed MSC and 5 × 10<sup>5</sup> EPC (EPC/MSC) that were mixed with β‐tricalcium phosphate (βTCP) scaffold. Domes filled with βTCP alone served as control. Rats were sacrificed after 4 or 12 weeks. Histomorphometry was used to determine blood vessel (Bv) density, vertical bone height, and bone area in the regenerated tissue.</p> </sec> <sec id="cid12104-sec-0004" sec-type="section"> <title>Results</title> <p>At both time points, new augmented hard tissue filled the space under the dome, and Bv density was higher in the EPC/MSC transplanted group vs control. However, bone height and bone area were similar among the groups 4 weeks posttransplantation, but were doubled in the EPC/MSC transplanted group 12 weeks posttransplantation.</p> </sec> <sec id="cid12104-sec-0005" sec-type="section"> <title>Conclusions</title> <p>EPC/MSC transplantation increases Bv formation in the early stages of healing that precedes enhancement of extracortical bone regeneration in later stages.</p> </sec> </abstract>