Formation and Abundance of 5‐Hydroxymethylcytosine in RNA. Issue 5 (12th February 2015)

• Record Type: Journal Article
• Authors: Huber, Sabrina M.; van Delft, Pieter; Mendil, Lee; Bachman, Martin; Smollett, Katherine; Werner, Finn; Miska, Eric A.; Balasubramanian, Shankar
• Is Part Of: Chembiochem. Volume 16:Issue 5(2015)
• Page Start: 752
• Page End: 755
• Publication Date: 2015-02-12
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201500013
• Languages: English
• ISSNs: 1439-4227

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>RNA methylation is emerging as a regulatory RNA modification that could have important roles in the control and coordination of gene transcription and protein translation. Herein, we describe an in vivo isotope‐tracing methodology to demonstrate that the ribonucleoside 5‐methylcytidine (m⁵C) is subject to oxidative processing in mammals, forming 5‐hydroxymethylcytidine (hm⁵C) and 5‐formylcytidine (f⁵C). Furthermore, we have identified hm⁵C in total RNA from all three domains of life and in polyA‐enriched RNA fractions from mammalian cells. This suggests m⁵C oxidation is a conserved process that could have critical regulatory functions inside cells.</p> </abstract>