Synthesis, characterization and in vitro evaluation of cytotoxicity and antimicrobial activity of chitosan–metal nanocomposites. Issue 5 (23rd April 2014)

• Record Type: Journal Article
• Authors: Kaur, Pawan; Thakur, Rajesh; Barnela, Manju; Chopra, Meenu; Manuja, Anju; Chaudhury, Ashok
• Is Part Of: Journal of chemical technology & biotechnology. Volume 90:Issue 5(2015:May)
• Page Start: 867
• Page End: 873
• Publication Date: 2014-04-23
• Subjects: Biotechnology -- Periodicals; Chemistry, Technical -- Periodicals; Chemical engineering -- Periodicals; Industries -- Environmental aspects -- Periodicals; 660
• DOI: 10.1002/jctb.4383
• Languages: English
• ISSNs: 0268-2575

Abstract

<abstract abstract-type="main" id="jctb4383-abs-0001"> <title>Abstract</title> <sec id="jctb4383-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p id="jctb4383-para-0001">The present study explores the synthesis of chitosan–metal nanocomposites in view of their increasing application as antimicrobial material.</p> </sec> <sec id="jctb4383-sec-0002" sec-type="section"> <title>RESULTS</title> <p id="jctb4383-para-0002">Chitosan nanoparticles were prepared by ionic gelation between chitosan and sodium tripolyphosphate. Copper sulphate hydrate (CuSO<sub>4</sub>.5H<sub>2</sub>O) and zinc acetate (Zn (O<sub>2</sub>CCH<sub>3</sub>)<sub>2</sub>) were used as precursors for synthesis of chitosan–copper nanocomposites (Cu/Ch) and chitosan–zinc nanocomposites (Zn/Ch), respectively. Synthesis of nanocomposites was confirmed by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy with energy dispersive X‐ray microanalyses (SEM‐EDX) and differential scanning calorimetry (DSC). Cytotoxicity of nanoformulations was studied by Resazurin assay on Vero cell line (African green monkey kidney cell line). Their antibacterial activities were assessed by the zone of inhibition method and time dependent growth curve against <italic>Micrococcus luteus</italic> MTCC 1809, <italic>Pseudomonas aeruginosa</italic> MTCC 424 and <italic>Salmonella enterica</italic> MTCC 1253 <italic>in vitro</italic>. Antifungal activity was also studied against <italic>Alternaria alterneta</italic>, <italic>Rhizoctonia solani</italic> and <italic>Aspergillus flavus in vitro</italic> by the mycelium inhibition method.</p> </sec> <sec id="jctb4383-sec-0003" sec-type="section"> <title>CONCLUSIONS</title> <p id="jctb4383-para-0003">It was observed that all nanoformulations show high antimicrobial activity against all test microorganisms. So chitosan–metal complexes could be promising candidates for novel antimicrobial agents in cosmetic, food and textile industries. © 2014 Society of Chemical Industry</p> </sec> </abstract>