Application of high‐throughput, high‐resolution and cost‐effective next generation sequencing‐based large‐scale HLA typing in donor registry. Issue 1 (24th November 2014) - Zhou, M.
Abstract Next generation sequencing (NGS)‐based human leukocyte antigen (HLA) typing was used for ultra large‐scale genotyping of registry donors for the China Marrow Donor Program (CMDP). More than 79, 000 samples were subjected to HLA genotyping at 4‐digit allelic level without ambiguities for HLA‐A, ‐B, ‐C, DRB1 and DQB1 loci, with throughput up to 2068 samples per lane in a HiSeq flow cell (eight lanes per run), and cost reduced by 95% compared with that of Sanger‐based typing. Two percent of randomly selected samples were quality control (QC) tested at 4‐digit allelic level by the CMDP QC laboratory, yielded a concordance of 99.72%. These results demonstrate that NGS is a cost effective and valuable tool for HLA typing of registry donors.