Temporal variation in mycorrhizal diversity and carbon and nitrogen stable isotope abundance in the wintergreen meadow orchid Anacamptis morio. Issue 3 (10th November 2014)

• Record Type: Journal Article
• Authors: Ercole, Enrico; Adamo, Martino; Rodda, Michele; Gebauer, Gerhard; Girlanda, Mariangela; Perotto, Silvia
• Is Part Of: New phytologist. Volume 205:Issue 3(2015)
• Page Start: 1308
• Page End: 1319
• Publication Date: 2014-11-10
• Subjects: Botany -- Periodicals; 580
• DOI: 10.1111/nph.13109
• Languages: English
• ISSNs: 0028-646X

Abstract

<abstract abstract-type="main" id="nph13109-abs-0001"> <title>Summary</title> <p> <list id="nph13109-list-0001" list-type="bullet"> <list-item> <p>Many adult orchids, especially photoautotrophic species, associate with a diverse range of mycorrhizal fungi, but little is known about the temporal changes that might occur in the diversity and functioning of orchid mycorrhiza during vegetative and reproductive plant growth.</p> </list-item> <list-item> <p>Temporal variations in the spectrum of mycorrhizal fungi and in stable isotope natural abundance were investigated in adult plants of <italic>Anacamptis morio</italic>, a wintergreen meadow orchid.</p> </list-item> <list-item> <p> <italic>Anacamptis morio</italic> associated with mycorrhizal fungi belonging to <italic>Tulasnella</italic>, <italic> Ceratobasidium</italic> and a clade of Pezizaceae (Ascomycetes). When a complete growing season was investigated, multivariate analyses indicated significant differences in the mycorrhizal fungal community. Among fungi identified from manually isolated pelotons, <italic>Tulasnella</italic> was more common in autumn and winter, the pezizacean clade was very frequent in spring, and <italic>Ceratobasidium</italic> was more frequent in summer. By contrast, relatively small variations were found in carbon (C) and nitrogen (N) stable isotope natural abundance, <italic>A. morio</italic> samples showing similar <sup>15</sup>N enrichment and <sup>13</sup>C depletion at the different sampling times.</p> </list-item> <list-item> <p>These observations suggest that, irrespective of differences in the seasonal environmental conditions, the plant phenological stages and the associated fungi, the isotopic content in mycorrhizal <italic>A. morio</italic> remains fairly constant over time.</p> </list-item> </list> </p> </abstract>