Fibroblast growth factor‐2 promotes healing of surgically created periodontal defects in rats with early, streptozotocin‐induced diabetes via increasing cell proliferation and regulating angiogenesis. (5th December 2014)

• Record Type: Journal Article
• Authors: Bizenjima, Takahiro; Seshima, Fumi; Ishizuka, Yoichi; Takeuchi, Takahiro; Kinumatsu, Takashi; Saito, Atsushi
• Is Part Of: Journal of clinical periodontology. Volume 42:Number 1(2015:Jan.)
• Page Start: 62
• Page End: 71
• Publication Date: 2014-12-05
• Subjects: Periodontics -- Periodicals; 617.6
• DOI: 10.1111/jcpe.12324
• Languages: English
• ISSNs: 0303-6979

Abstract

<abstract abstract-type="main" id="jcpe12324-abs-0001"> <title>Abstract</title> <sec id="jcpe12324-sec-0001" sec-type="section"> <title>Aim</title> <p>To evaluate the effects of fibroblast growth factor (FGF)‐2 on the healing of surgical periodontal defects in rats with early, streptozotocin‐induced diabetes.</p> </sec> <sec id="jcpe12324-sec-0002" sec-type="section"> <title>Materials and Methods</title> <p>Fifty Wistar rats were assigned to streptozotocin‐induced diabetes or non‐diabetes group. Periodontal defects were surgically created at maxillary first molars. Defects were treated with hydroxypropyl cellulose (HPC) or FGF‐2 with HPC. Defect fill was evaluated by microcomputed tomography. Histological and immunohistochemical analyses were performed.</p> </sec> <sec id="jcpe12324-sec-0003" sec-type="section"> <title>Results</title> <p>Compared to vehicle alone, FGF‐2 treatment yielded significantly greater bone volume and trabecular thickness in diabetes group. Diabetes group displayed reduced new bone formation and significantly longer epithelial down‐growth compared to non‐diabetes group. In diabetes group, FGF‐2 treatment increased PCNA‐positive cells and new bone formation after 2 weeks and suppressed epithelial down‐growth, but new cementum formation was minimal even after 4 weeks. In diabetes group, overexpression of vascular endothelial growth factor was evident in cells within connective tissue, and no significant enhancement was observed by FGF‐2 treatment. FGF‐2 increased the expression of <italic>α</italic>‐smooth muscle actin in diabetes group.</p> </sec> <sec id="jcpe12324-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Treatment of surgical periodontal defects in diabetic rats with the single application of FGF‐2 provided beneficial effects primarily on new bone formation via increasing cell proliferation and regulating angiogenesis.</p> </sec> </abstract>