Deletion mapping in the Enhancer of split complex. Issue 6 (December 2014)

• Record Type: Journal Article
• Authors: Wurmbach, Elisa; Preiss, Anette
• Is Part Of: Hereditas. Volume 151:Issue 6(2014:Dec.)
• Page Start: 159
• Page End: 168
• Publication Date: 2014-12
• Subjects: Heredity -- Periodicals; Genetics -- Periodicals; Cytogénétique; Génétique; Hérédité; Périodique électronique (Descripteur de forme); Ressource Internet (Descripteur de forme); 576.5
• DOI: 10.1111/hrd2.00065
• Languages: English
• ISSNs: 0018-0661

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The <italic>Enhancer of split complex [E(spl)‐C]</italic> comprises twelve genes of different classes. Seven genes encode proteins of with a basic‐helix‐loop‐helix‐orange (bHLH‐O) domain that function as transcriptional repressors and serve as effectors of the Notch signalling pathway. They have been named <italic>E(spl)m8‐, m7‐, m5‐, m3‐, mβ‐, mγ‐</italic> and <italic>mδ‐HLH</italic>. Four genes, <italic>E(spl)m6‐, m4‐, m2‐</italic> and <italic>mα‐BFM</italic> are intermingled and encode Notch repressor proteins of the Bearded‐family (BFM). The complex is split by a single gene of unrelated function, encoding a Kazal‐type protease inhibitor (<italic>Kaz‐m1</italic>). All members within a family, bHLH‐O or BFM, are very similar in structure and in function. In an attempt to generate specific mutants, we have mobilised P‐element constructs residing next to <italic>E(spl)m7‐HLH</italic> and <italic>E(spl)mγ‐HLH</italic>, respectively. The resulting deletions were mapped molecularly and by cytology. Two small deletions affected only <italic>E(spl)m7‐HLH</italic> and <italic>E(spl)mδ</italic>. The deficient flies were viable without apparent phenotype. Larger deletions, generated also by X‐ray mutagenesis, uncover most of the <italic>E(spl)‐C</italic>. The phenotypes of homozygous deficient embryos were analysed to characterize the respective loss of Notch signalling activity.</p> </abstract>