Arabidopsis DOK1 encodes a functional dolichol kinase involved in reproduction. (16th December 2014)

• Record Type: Journal Article
• Authors: Kanehara, Kazue; Cho, Yueh; Lin, Ying‐Chen; Chen, Chia‐En; Yu, Chao‐Yuan; Nakamura, Yuki
• Is Part Of: Plant journal. Volume 81:Number 2(2015:Jan.)
• Page Start: 292
• Page End: 303
• Publication Date: 2014-12-16
• Subjects: Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.12727
• Languages: English
• ISSNs: 0960-7412

Abstract

<abstract abstract-type="main" id="tpj12727-abs-0001"> <title>Summary</title> <p>Dolichol phosphate (Dol‐P) serves as a carrier of complex polysaccharides during protein glycosylation. Dol‐P is synthesized by the phosphorylation of dolichol or the monodephosphorylation of dolichol pyrophosphate (Dol‐PP); however, the enzymes that catalyze these reactions remain unidentified in <italic>Arabidopsis thaliana</italic>. We performed a genome‐wide search for cytidylyltransferase motif‐containing proteins in Arabidopsis, and found that At3g45040 encodes a protein homologous with Sec59p, a dolichol kinase (DOK) in <italic>Saccharomyces cerevisiae</italic>. At3g45040, designated At<italic>DOK1</italic>, complemented defects in the growth and <italic>N</italic>‐linked glycosylation of the <italic>S. cerevisiae sec59</italic> mutant, suggesting that At<italic>DOK1</italic> encodes a functional DOK. To characterize the physiological roles of AtDOK1 <italic>in planta</italic>, we isolated two independent lines of T‐DNA‐tagged At<italic>DOK1</italic> mutants, <italic>dok1</italic>‐<italic>1</italic> and <italic>dok1</italic>‐<italic>2</italic>. The heterozygous plants showed developmental defects in male and female gametophytes, including an aberrant pollen structure, low pollen viability, and short siliques. Additionally, the mutations had incomplete penetrance. These results suggest that AtDOK1 is a functional DOK required for reproductive processes in Arabidopsis.</p> </abstract>