Characterization of the novel Sezary lymphoma cell line BKP1. Issue 1 (13th November 2014)

• Record Type: Journal Article
• Authors: Boudjarane, John; Essaydi, Arnaud; Farnault, Laure; Popovici, Cornel; Lafage‐Pochitaloff, Marina; Beaufils, Nathalie; Berda‐Haddad, Yaël; Lacroix, Romaric; Nicolino‐Brunet, Corinne; Le Treut, Thérèse; Zattara, Hélène; Gabert, Jean; Kahn‐Perlès, Brigitte; Costello, Régis
• Is Part Of: Experimental dermatology. Volume 24:Issue 1(2015:Jan.)
• Page Start: 60
• Page End: 62
• Publication Date: 2014-11-13
• Subjects: Dermatology -- Periodicals; 616.5
• DOI: 10.1111/exd.12567
• Languages: English
• ISSNs: 0906-6705

Abstract

<abstract abstract-type="main" id="exd12567-abs-0001"> <title>Abstract</title> <p>Cutaneous T‐cell lymphomas (CTCL) are a heterogeneous group of lymphomas primarily involving the skin. The most common types are mycosis fungoides (MF) and Sezary Syndrome (SS). We report a novel long‐term fast‐growing SS line termed BKP1 that was characterized by flow cytometry (FC), conventional and molecular cytogenetic [FISH/multi‐FISH together with array comparative genomic hybridization (aCGH)]. FC immunophenotype of the BKP1 is CD2+CD5+CD3+CD4+CD8−CD7−CD25−CD26−CD30−CD158k+. The TCR<italic>γ</italic> characterization of BKP1 by PCR identified a clonal rearrangement. The conventional cytogenetic and Multi‐FISH analysis showed complex chromosomal rearrangements. aCGH analysis highlighted the loss of genes involved in cell cycle control, in immune response (<italic>HLA</italic>, complement complex) and DNA damage repair mechanisms. The BKP1 is another lymphoma cell line thoroughly characterized that can be a valuable tool for both basic and applied research such as identification of deregulated genes and/or pathways and screening for new antilymphoma drugs.</p> </abstract>