Ligand‐Detected Relaxation Dispersion NMR Spectroscopy: Dynamics of preQ1–RNA Binding1. Issue 2 (17th November 2014)

• Record Type: Journal Article
• Authors: Moschen, Thomas; Wunderlich, Christoph Hermann; Spitzer, Romana; Levic, Jasmin; Micura, Ronald; Tollinger, Martin; Kreutz, Christoph
• Is Part Of: Angewandte Chemie international edition. Volume 54:Issue 2(2015)
• Page Start: 560
• Page End: 563
• Publication Date: 2014-11-17
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201409779
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>An NMR‐based approach to characterizing the binding kinetics of ligand molecules to biomolecules, like RNA or proteins, by ligand‐detected Carr‐Purcell‐Meiboom‐Gill (CPMG) relaxation dispersion experiments is described. A ¹⁵N‐modified preQ₁ ligand is used to acquire relaxation dispersion experiments in the presence of low amounts of the <italic>Fsu</italic> class I preQ₁ aptamer RNA, and increasing ligand concentrations to probe the RNA small molecule interaction. Our experimental data strongly support the conformational selection mechanism postulated. The approach gives direct access to two parameters of a ligand–receptor interaction: the off rate and the population of the small molecule–receptor complex. A detailed description of the kinetics underlying the ligand binding process is of crucial importance to fully understanding a riboswitch's function and to evaluate potential new antibiotics candidates targeting the noncoding RNA species. Ligand‐detected NMR relaxation dispersion experiments represent a valuable diagnostic tool for the characterization of binding mechanisms.</p> </abstract>