Indicator Displacement Assays Inside Live Cells1. Issue 3 (27th November 2014)

• Record Type: Journal Article
• Authors: Norouzy, Amir; Azizi, Zahra; Nau, Werner M.
• Is Part Of: Angewandte Chemie international edition. Volume 54:Issue 3(2015)
• Page Start: 792
• Page End: 795
• Publication Date: 2014-11-27
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201407808
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>The macrocycle <italic>p</italic>‐sulfonatocalix[4]arene (CX4) and the fluorescent dye lucigenin (LCG) form a stable host–guest complex, in which the dye fluorescence is quenched. Incubation of live V79 and CHO cells with the CX4/LCG chemosensing ensemble resulted in its spontaneous uptake. Subsequent addition of choline, acetylcholine, or protamine, which have a high affinity for CX4 and are capable of entering cells, resulted in a fluorescence switch‐on response. This can be traced to the displacement of LCG from CX4 by the analytes. The results establish the principal functionality of indicator displacement assays with synthetic receptors for the detection of the uptake of bioorganic analytes by live cells.</p> </abstract>