Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes. (February 2015)

• Record Type: Journal Article
• Authors: Salamanna, F.; Frizziero, A.; Pagani, S.; Giavaresi, G.; Curzi, D.; Falcieri, E.; Marini, M.; Abruzzo, P. M.; Martini, L.; Fini, M.
• Is Part Of: Connective tissue research. Volume 56:Number 1(2015:Feb.)
• Page Start: 35
• Page End: 43
• Publication Date: 2015-02
• Subjects: Connective tissues -- Periodicals; 616.770072
• DOI: 10.3109/03008207.2014.979166
• Languages: English
• ISSNs: 0300-8207

Abstract

<abstract> <title>Abstract</title> <p>The purpose of this study was to investigate tenocyte mechanobiology after sudden-detraining and to examine the hypothesis that repeated peri-patellar injections of hyaluronic acid (HA) on detrained patellar tendon (PT) may reduce and limit detrained-associated damage in tenoctyes.</p> <p>Twenty-four male Sprague–Dawley rats were divided into three groups: Untrained, Trained and Detrained. In the Detrained rats, the left tendon was untreated while the right tendon received repeated peri-patellar injections of either HA or saline (NaCl). Tenocyte morphology, metabolism and synthesis of C-terminal-propeptide of type I collagen, collagen-III, fibronectin, aggrecan, tenascin-c, interleukin-1β, matrix-metalloproteinase-1 and-3 were evaluated after 1, 3, 7 and 10 days of culture. Transmission-electronic-microscopy showed a significant increase in mitochondria and rough endoplasmic reticulum in cultured tenocytes from Detrained-HA with respect to those from Detrained-NaCl. Additionally, Detrained-HA cultures showed a significantly higher proliferation rate and viability, and increased synthesis of C-terminal-Propeptide of type I collagen, fibronectin, aggrecan, tenascin-c and matrix-metalloproteinase-3 with respect to Detrained-NaCl ones, whereas synthesis of matrix-metalloproteinase-1 and interleukin-1β was decreased. Our study demonstrates that discontinuing training activity in the short-term alters tenocyte synthetic and metabolic activity and that repeated peri-patellar infiltrations of HA during detraining allow the maintenance of tenocyte anabolic activity.</p> </abstract>