Characterization of the first report of Mycobacterium timonense infecting an HIV patient in an Ecuadorian hospital. (16th July 2014)

• Record Type: Journal Article
• Authors: Zurita, J.; Ortega‐Paredes, D.; Mora, M.; Espinel, N.; Parra, H.; Febres, L.; Zurita‐Salinas, C.; Drancourt, M.
• Is Part Of: Clinical microbiology and infection. Volume 20:Number 12(2014:Dec.)
• Page Start: O1113
• Page End: O1116
• Publication Date: 2014-07-16
• Subjects: Medical microbiology -- Periodicals; Diagnostic microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 616.01
• DOI: 10.1111/1469-0691.12675
• Languages: English
• ISSNs: 1198-743X

Abstract

<abstract abstract-type="main" id="clm12675-abs-0001"> <title>Abstract</title> <p> <italic>Mycobacterium timonense</italic> is a non‐tuberculous mycobacteria (NTM) described in southern France in 2009, and to our knowledge, not reported again as a human pathogen in indexed literature. The aim of this work was to characterize the first clinical isolate of <italic>M. timonense</italic> in Ecuador. Time of growth, biochemical tests, thin layer growth test, PCR‐RFLP analysis of the <italic>hsp65</italic> gene and MALDI‐TOF spectra analysis were not able to identify the species. The species identification was achieved through sequencing of <italic>rrs</italic>, <italic> hsp65</italic> and <italic>rpoB</italic> genes. The results highlight the necessity to set up a sequencing method to identify emerging NTM in Ecuadorian clinical facilities.</p> </abstract>