Biomechanical changes from long‐term freezer storage and cellular reduction of tracheal scaffoldings. (5th August 2014)

• Record Type: Journal Article
• Authors: Jones, Matthew C.; Rueggeberg, Frederick A.; Cunningham, Aaron J.; Faircloth, Hunter A.; Jana, Tanima; Mettenburg, Donald; Waller, Jennifer L.; Postma, Gregory N.; Weinberger, Paul M.
• Is Part Of: Laryngoscope. Volume 125:Number 1(2015:Jan.)
• Page Start: E16
• Page End: E22
• Publication Date: 2014-08-05
• Subjects: Otolaryngology -- Periodicals; 617.51005
• DOI: 10.1002/lary.24853
• Languages: English
• ISSNs: 0023-852X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="lary24853-sec-0001" sec-type="section"> <title>Objectives/Hypothesis</title> <p>To determine structural biomechanical changes in tracheal scaffolds resulting from cellular reduction and storage at −80<sup>o</sup>C.</p> </sec> <sec id="lary24853-sec-0002" sec-type="section"> <title>Study Design</title> <p>Laboratory‐based study.</p> </sec> <sec id="lary24853-sec-0003" sec-type="section"> <title>Methods</title> <p>Forty‐four rabbit tracheal segments were separated into four treatment groups: untreated (group A, control), cellular‐reduced (group B), storage at −80<sup>o</sup>C followed by cellular reduction (group C), and cellular‐reduced followed by storage at −80<sup>o</sup>C (group D). Tracheal segments were subjected to uniaxial tension (n = 21) or compression (n = 23) using a universal testing machine to determine sutured tensile yield load and radial compressive strengths at 50% lumen occlusion. Mean differences among groups for tension and compression were compared by analysis of variance with post‐hoc Tukey‐Kramer test.</p> </sec> <sec id="lary24853-sec-0004" sec-type="section"> <title>Results</title> <p>The untreated trachea (group A) demonstrated mean yield strength of 5.93 (± 1.65) N and compressive strength of 2.10 (± 0.51) N. Following treatment/storage, the tensile yield strength was not impaired (group B = 6.79 [± 1.58] N, C = 6.21 [± 1.40] N, D = 6.26 [± 1.18]; <italic>P</italic> > 0.10 each). Following cellular reduction, there was a significant reduction in compressive strength (group B = 0.44 N [± 0.13], <italic>P</italic> < 0.0001), but no further reduction due to storage (group C = 0.39 N [± 0.10]; <italic>P</italic> = 0.97 compared to group B).</p> </sec> <sec id="lary24853-sec-0005" sec-type="section"> <title>Conclusion</title> <p>The data suggest cellular reduction leads to loss of compressive strength. Freezing at −80°C (either before, or subsequent to cellular reduction) may be a viable storage method for tracheal grafts.</p> </sec> <sec id="lary24853-sec-0006" sec-type="section"> <title>Level of Evidence</title> <p>N/A. <italic>Laryngoscope</italic>, 125:E16–E22, 2015</p> </sec> </abstract>