Simultaneous analysis of seven 2‐hydroxy fatty acids as tert‐butyldimethylsilyl derivatives in plasma by gas chromatography–mass spectrometry. (5th June 2014)

• Record Type: Journal Article
• Authors: Seo, Chan; Yoon, Jaehwan; Rhee, Yumie; Kim, Jong‐Jin; Nam, Sang‐Jip; Lee, Wonjae; Lee, Gwang; Yee, Sung‐Tae; Paik, Man‐Jeong
• Is Part Of: Biomedical chromatography. Volume 29:Number 1(2015:Jan.)
• Page Start: 156
• Page End: 160
• Publication Date: 2014-06-05
• Subjects: Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3251
• Languages: English
• ISSNs: 0269-3879

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>An efficient method for the simultaneous analysis of seven 2‐hydroxy fatty acids (2‐HFAs) as <italic>tert</italic>‐butyldimethylsilyl (TBDMS) derivative was developed by gas chromatography–mass spectrometry in selected ion monitoring mode. New mass spectral data on 2‐hydroxycapric, 2‐hydroxypalmitic, 2‐hydroxystearic and 2‐hydroxybehenic acids as <italic>di</italic>‐TBDMS derivatives for hydroxyl and carboxyl groups were built. Under the optimal conditions, the present method showed a good correlation coefficient (<italic>r</italic> ≥ 0.999) in the range of 0.01–0.5 µg. The precision showed low relative standard deviation of &lt;10%, and the accuracy (percentage relative error) varied from −5.2 to 0.3 for the seven 2‐HFAs studied. Recovery rates of all 2‐HFAs were ≥ 93.2% with good precision. When applied to normal human plasma, seven 2‐HFAs were positively identified. Therefore, the present efficient method will be useful for simultaneous analysis of 2‐HFAs in plasma. Copyright © 2014 John Wiley &amp; Sons, Ltd.</p> </abstract>