Biological parameters influencing the human umbilical cord‐derived mesenchymal stem cells' response to retinoic acid. (19th November 2014)

• Record Type: Journal Article
• Authors: Saraee, Farnoosh; Sagha, Mohsen; Mohseni Kouchesfehani, Homa; Abdanipour, Alireza; Maleki, Masoud; Nikougoftar, Mahin
• Is Part Of: BioFactors. Volume 40:Number 6(2014:Nov./Dec.)
• Page Start: 624
• Page End: 635
• Publication Date: 2014-11-19
• Subjects: Vitamins -- Physiological effect -- Periodicals; Trace elements -- Physiological effect -- Periodicals; Growth factors -- Physiological effect -- Periodicals; Plant growth promoting substances -- Physiological effect -- Periodicals; Biochemistry -- Periodicals; Nutritional Physiological Phenomena -- Periodicals; Trace Elements -- metabolism -- Periodicals; Vitamins -- metabolism -- Periodicals; Molecular Biology -- Periodicals; 612.399
• DOI: 10.1002/biof.1192
• Languages: English
• ISSNs: 0951-6433

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Human umbilical cord‐derived mesenchymal stem cells (HUCMSCs) are multipotent fetal stem cells that differentiate into various cell lineages. In recent years, they have gained attention for therapeutic applications but very little is known about their sensitivity to chemical agents such as widely used retinoic acid (RA). As a morphogen inducing differentiation of mesenchymal stem cells, RA has for a long time been known to be a potent teratogen promoting craniofacial and limb abnormality in vertebrate embryos. Here, using MTT assay and EB/AO staining as well as TUNEL assay we show that RA in a concentration‐dependent manner induces apoptosis through upregulating <italic>Caspase</italic> expression and increasing <italic>Bax/Bcl2</italic> ratio. Moreover, different biological parameters such as initial time seeding, cell density, passage number and duration of RA treatment play a major role in HUCMSCs cytotoxic response to this agent. © 2014 BioFactors, 40(6):624–635, 2014</p> </abstract>