Comparison of in vivo and in vitro digestion on polyphenol composition in lingonberries: Potential impact on colonic health. (30th October 2014)

• Record Type: Journal Article
• Authors: Brown, Emma M.; Nitecki, Sonja; Pereira‐Caro, Gema; McDougall, Gordon J.; Stewart, Derek; Rowland, Ian; Crozier, Alan; Gill, Chris I.R.
• Is Part Of: BioFactors. Volume 40:Number 6(2014:Nov./Dec.)
• Page Start: 611
• Page End: 623
• Publication Date: 2014-10-30
• Subjects: Vitamins -- Physiological effect -- Periodicals; Trace elements -- Physiological effect -- Periodicals; Growth factors -- Physiological effect -- Periodicals; Plant growth promoting substances -- Physiological effect -- Periodicals; Biochemistry -- Periodicals; Nutritional Physiological Phenomena -- Periodicals; Trace Elements -- metabolism -- Periodicals; Vitamins -- metabolism -- Periodicals; Molecular Biology -- Periodicals; 612.399
• DOI: 10.1002/biof.1173
• Languages: English
• ISSNs: 0951-6433

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>The composition of polyphenols in ileal fluid samples obtained from an ileostomy subject after lingonberry intake was compared with lingonberry extracts obtained after simulated <italic>in vitro</italic> digestion (IVDL) and subsequent faecal fermentation (IVFL). HPLC‐PDA‐MS/MS analysis confirmed similar patterns of lingonberry (poly)phenolic metabolism after the <italic>in vivo</italic> and <italic>in vitro</italic> digestion, with reduced recovery of anthocyanins and a similar pattern of recovery for proanthocyanidins observed for both methods of digestion. On the other hand, the IVFL sample contained none of the original (poly)phenolic components but was enriched in simple aromatic components. Digested and fermented extracts exhibited significant (<italic>P <</italic> 0.05) anti‐genotoxic (Comet assay), anti‐mutagenic (Mutation Frequency assay), and anti‐invasive (Matrigel Invasion assay) effects in human cell culture models of colorectal cancer at physiologically‐relevant doses (0–50 μg/mL gallic acid equivalents). The ileal fluid induced significant anti‐genotoxic activity (<italic>P <</italic> 0.05), but at a higher concentration (200 μg/mL gallic acid equivalents) than the IVDL. Despite extensive structural modification following digestion and fermentation, lingonberry extracts retained their bioactivity <italic>in vitro</italic>. This reinforces the need for studies to consider the impact of digestion when investigating bioactivity of dietary phytochemicals. © 2014 BioFactors, 40(6):611–623, 2014</p> </abstract>