Persistence of recipient‐derived as well as donor‐derived clones of cytomegalovirus pp65‐specific cytotoxic T cells long after allogeneic hematopoietic stem cell transplantation. Issue 6 (28th November 2014)

• Record Type: Journal Article
• Authors: Terasako‐Saito, K.; Nakasone, H.; Tanaka, Y.; Yamazaki, R.; Sato, M.; Sakamoto, K.; Ishihara, Y.; Kawamura, K.; Akahoshi, Y.; Hayakawa, J.; Wada, H.; Harada, N.; Nakano, H.; Kameda, K.; Ugai, T.; Yamasaki, R.; Ashizawa, M.; Kimura, S.‐I.; Kikuchi, M.; Tanihara, A.; Kanda, J.; Kako, S.; Nishida, J.; Kanda, Y.
• Is Part Of: Transplant infectious disease. Volume 16:Issue 6(2014)
• Page Start: 930
• Page End: 940
• Publication Date: 2014-11-28
• Subjects: Transplantation of organs, tissues, etc -- Complications -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 617.01
• DOI: 10.1111/tid.12318
• Languages: English
• ISSNs: 1398-2273

Abstract

<abstract abstract-type="main" id="tid12318-abs-0001"> <title>Abstract</title> <sec id="tid12318-sec-0001" sec-type="section"> <title>Background</title> <p>Cytomegalovirus (CMV)‐specific CD8<sup>+</sup> cytotoxic T lymphocytes (CMV‐CTLs) play a crucial role in preventing CMV disease. However, the actual <italic>in vivo</italic> dynamics of CMV‐CTL clones after allogeneic hematopoietic stem cell transplantation (alloHCT) are still unclear.</p> </sec> <sec id="tid12318-sec-0002" sec-type="section"> <title>Methods</title> <p>Using a single‐cell T‐cell receptor repertoire analysis, we monitored clones and chimerism of CMV‐CTLs in 3 CMV‐seropositive alloHCT recipients from CMV‐seronegative donors, with or without CMV reactivation.</p> </sec> <sec id="tid12318-sec-0003" sec-type="section"> <title>Results</title> <p>Nearly all of the CMV‐CTLs during follow‐up were CD45RA<sup>−</sup>CCR7<sup>−</sup> effector memory/CD45RA<sup>+</sup>CCR7<sup>−</sup> effector T cells, and were highly matured. In each case, the use of BV gene families was restricted, especially in BV5, 7, 28, and 29. Although no common predominant CMV‐CTL clones were found, several shared motifs of complementarity‐determining region‐3 were identified among the 3 cases; QGA in all, TGE and TDT in Case 1 and Case 2, and RDRG in Case 2 and Case 3. In all cases, CMV‐CTL clones that were detected for the first time after alloHCT persisted as the dominant clones. In Case 1, without CMV reactivation, recipient‐derived CMV‐CTLs exclusively persisted as a dominant clone, while all CMV‐CTLs in the other 2 cases, with CMV reactivation, were donor derived.</p> </sec> <sec id="tid12318-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Clone monitoring and chimerism analyses should help to further clarify novel aspects of immuno‐reconstitution after alloHCT.</p> </sec> </abstract>