Allotype analysis to determine the origin of cytomegalovirus immunoglobulin‐G after allogeneic stem cell transplantation. Issue 6 (4th November 2014)

• Record Type: Journal Article
• Authors: Yamazaki, R.; Tanaka, Y.; Nakasone, H.; Sato, M.; Terasako‐Saito, K.; Sakamoto, K.; Akahoshi, Y.; Nakano, H.; Ugai, T.; Yamasaki, R.; Wada, H.; Ishihara, Y.; Kawamura, K.; Ashizawa, M.; Kimura, S.‐I.; Kikuchi, M.; Kako, S.; Kanda, J.; Tanihara, A.; Nishida, J.; Kanda, Y.
• Is Part Of: Transplant infectious disease. Volume 16:Issue 6(2014)
• Page Start: 904
• Page End: 913
• Publication Date: 2014-11-04
• Subjects: Transplantation of organs, tissues, etc -- Complications -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 617.01
• DOI: 10.1111/tid.12304
• Languages: English
• ISSNs: 1398-2273

Abstract

<abstract abstract-type="main" id="tid12304-abs-0001"> <title>Abstract</title> <sec id="tid12304-sec-0001" sec-type="section"> <title>Background</title> <p>Cytomegalovirus (CMV) reactivation still remains a major problem following allogeneic hematopoietic stem cell transplantation (HSCT).</p> </sec> <sec id="tid12304-sec-0002" sec-type="section"> <title>Patients and methods</title> <p>In this study, we analyzed an immunoglobulin allotype, IgG1m(f), in CMV‐seropositive HSCT recipients and their donors to distinguish donor‐derived antibody from recipient‐derived antibody. Eight donor‐recipient pairs were informative regarding the appearance of donor‐derived immunoglobulin‐G (IgG), as the recipients were homozygous null for the IgG1m(f) allotype and the donors were IgG1m(f) positive. In these patients, total IgG, IgM, and allotype‐specific IgG against CMV were measured by enzyme‐linked immunosorbent assay. All subjects were monitored for at least 9 months after HSCT with (<italic>n</italic> = 5) or without (<italic>n</italic> = 3) CMV reactivation.</p> </sec> <sec id="tid12304-sec-0003" sec-type="section"> <title>Results</title> <p>Donor‐derived CMV IgG tended to be elevated earlier in patients with CMV‐seropositive donors than in those with CMV‐seronegative donors. In 1 patient with a CMV‐negative donor, donor‐derived CMV IgG was not detected until late CMV reactivation. In 3 patients without CMV reactivation, donor‐derived CMV IgG was also elevated within 1–6 months after HSCT.</p> </sec> <sec id="tid12304-sec-0004" sec-type="section"> <title>Conclusion</title> <p>In conclusion, the CMV serostatus of the donor may be related to the timing of the appearance of donor‐derived CMV IgG and the reconstitution of humoral immunity against CMV, regardless of the CMV antigenemia level after HSCT.</p> </sec> </abstract>