The Coiled‐Coil Domain Containing 80 (ccdc80) Gene Regulates gadd45β2 Expression in the Developing Somites of Zebrafish as a New Player of the Hedgehog Pathway. Issue 4 (April 2015)

• Record Type: Journal Article
• Authors: Della Noce, Isabella; Carra, Silvia; Brusegan, Chiara; Critelli, Rosina; Frassine, Andrea; De Lorenzo, Carlo; Giordano, Antonio; Bellipanni, Gianfranco; Villa, Erica; Cotelli, Franco; Pistocchi, Anna; Schepis, Filippo
• Is Part Of: Journal of cellular physiology. Volume 230:Issue 4(2015:Apr.)
• Page Start: 821
• Page End: 830
• Publication Date: 2015-04
• Subjects: Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.24810
• Languages: English
• ISSNs: 0021-9541

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jcp24810-sec-0001" sec-type="section"> <p>The <italic>Coiled‐Coil Domain Containing 80</italic> (<italic>CCDC80</italic>) gene has been identified as strongly induced in rat thyroid PC CL3 cells immortalized by the adenoviral E1A gene. In human, <italic>CCDC80</italic> is a potential oncosoppressor due to its down‐regulation in several tumor cell lines and tissues and it is expressed in almost all tissues. <italic>CCDC80</italic> has homologous in mouse, chicken, and zebrafish. We cloned the zebrafish <italic>ccdc80</italic> and analyzed its expression and function during embryonic development. The in‐silico translated zebrafish protein shares high similarity with its mammalian homologous, with nuclear localization signals and a signal peptide. Gene expression analysis demonstrates that zebrafish <italic>ccdc80</italic> is maternally and zygotically expressed throughout the development. In particular, <italic>ccdc80</italic> is strongly expressed in the notochord and it is under the regulation of the <italic>Hedgehog</italic> pathway. In this work we investigated the functional effects of <italic>ccdc80</italic>‐loss‐of‐function during embryonic development and verified its interaction with <italic>gadd45β2</italic> in somitogenesis. J. Cell. Physiol. 230: 821–830, 2015. © 2014 Wiley Periodicals, Inc.</p> </sec> </abstract>