Systemic administration of valproic acid stimulates overexpression of microtubule-associated protein 2 in the spinal cord injury model to promote neurite outgrowth. (March 2015)

• Record Type: Journal Article
• Authors: Abdanipour, Alireza; Schluesener, Hermann J.; Tiraihi, Taki; Noori-Zadeh, Ali
• Is Part Of: Neurological research. Volume 37:Number 3(2015)
• Page Start: 223
• Page End: 228
• Publication Date: 2015-03
• Subjects: Neurology -- Periodicals; Neurosciences -- Periodicals; 616.8005
• DOI: 10.1179/1743132814Y.0000000438
• Languages: English
• ISSNs: 0161-6412

Abstract

<abstract> <title> <x content-type="archive" xml:space="preserve">Abstract</x> </title> <sec> <title>Objectives:</title> <p>Growing body of evidence suggests that neurite outgrowth is a key determinant in the re-networking of damaged neuronal circuits as well as synaptogenesis. The essential molecule in this interesting process is microtubule-associated protein 2 (MAP2) studies demonstrated that inhibition of MAP2 by antisense Oligonucleotide hinders neurite outgrowth.</p> </sec> <sec> <title>Methods:</title> <p>In the current study, we evaluated the effects of valproic acid (VPA), a histone deacetylase inhibitor on the expression of MAP2 in the rat spinal cord injury model by real time RT-PCR and immunoreactivity assays.</p> </sec> <sec> <title>Results:</title> <p>We revealed that a significant increase in the MAP2 overexpression occurred in VPA-treated group compared to the sham group by quantative real-time reverse transcription polymerase chain reaction (RT-PCR) and immunoreactivity assays for MAP2 gene expression.</p> </sec> <sec> <title>Discussion:</title> <p>The present findings indicated that systemic administration of VPA stimulated MAP2 gene expression and also supports the involvement of the MAP2-mediated <italic>de novo</italic> re-arborization and neurite outgrowth of neurons, which might contribute to successful neuronal re-wirings. Thus, VPA has promising applications in the treatment of many neurological problems, such as neurotrauma, Alzheimer and Parkinson's disease, and others.</p> </sec> </abstract>