OsLOL1, a C2C2‐type zinc finger protein, interacts with OsbZIP58 to promote seed germination through the modulation of gibberellin biosynthesis in Oryza sativa. (December 2014)

• Record Type: Journal Article
• Authors: Wu, Jiahe; Zhu, Chuanfeng; Pang, Jinhuan; Zhang, Xiangrong; Yang, Chunlin; Xia, Guixian; Tian, Yingchuan; He, Chaozu
• Is Part Of: Plant journal. Volume 80:Number 6(2014:Dec.)
• Page Start: 1118
• Page End: 1130
• Publication Date: 2014-12
• Subjects: Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.12714
• Languages: English
• ISSNs: 0960-7412

Abstract

<abstract abstract-type="main" id="tpj12714-abs-0001"> <title>Summary</title> <p>Seed germination is a key developmental process in the plant life cycle that is influenced by various environmental cues and phytohormones through gene expression and a series of metabolism pathways. In the present study, we investigated a C2C2‐type finger protein, OsLOL1, which promotes gibberellin (GA) biosynthesis and affects seed germination in <italic>Oryza sativa</italic> (rice). We used OsLOL1 antisense and sense transgenic lines to explore OsLOL1 functions. Seed germination timing in antisense plants was restored to wild type when exogenous GA<sub>3</sub> was applied. The reduced expression of the GA biosynthesis gene <italic>OsKO2</italic> and the accumulation of <italic>ent</italic>‐kaurene were observed during germination in antisense plants. Based on yeast two‐hybrid and firefly luciferase complementation analyses, OsLOL1 interacted with the basic leucine zipper protein OsbZIP58. The results from electrophoretic mobility shift and dual‐luciferase reporter assays showed that OsbZIP58 binds the G‐box <italic>cis</italic>‐element of the <italic>OsKO2</italic> promoter and activates LUC reporter gene expression, and that interaction between OsLOL1 and OsbZIP58 activates <italic>OsKO2</italic> gene expression. In addition, OsLOL1 decreased <italic>SOD1</italic> gene expression and accelerated programmed cell death (PCD) in the aleurone layer of rice grains. These findings demonstrate that the interaction between OsLOL1 and OsbZIP58 influences GA biosynthesis through the activation of <italic>OsKO2</italic> via OsbZIP58, thereby stimulating aleurone PCD and seed germination.</p> </abstract>