Increased D‐serine in the aqueous and vitreous humour in patients with proliferative diabetic retinopathy. (8th May 2014)

• Record Type: Journal Article
• Authors: Jiang, Haiyan; Du, Jinlin; He, Tao; Qu, Jia; Song, Zongming; Wu, Shengzhou
• Is Part Of: Clinical & experimental ophthalmology. Volume 42:Number 9(2014)
• Page Start: 841
• Page End: 845
• Publication Date: 2014-05-08
• Subjects: Ophthalmology -- Periodicals; 617.7
• DOI: 10.1111/ceo.12329
• Languages: English
• ISSNs: 1442-6404

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="ceo12329-sec-0001" sec-type="section"> <title>Background</title> <p>The objective of this clinical study is to examine the association between D‐serine and diabetic retinopathy (DR).</p> </sec> <sec id="ceo12329-sec-0002" sec-type="section"> <title>Design</title> <p>Retrospective, case‐control study was performed in the affiliated Eye Hospital of Wenzhou Medical University.</p> </sec> <sec id="ceo12329-sec-0003" sec-type="section"> <title>Participants</title> <p>This study included 25 patients with proliferative diabetic retinopathy (PDR), and 25 sex‐ and age‐matched control subjects, i.e. patients with idiopathic macular hole and idiopathic epiretinal membrane.</p> </sec> <sec id="ceo12329-sec-0004" sec-type="section"> <title>Methods</title> <p>Clinical diagnoses were made by the senior ophthalmologists in the Eye Hospital; the aqueous and vitreous humour specimens were collected from these patients undergoing pars plana vitrectomy for treating complications.</p> </sec> <sec id="ceo12329-sec-0005" sec-type="section"> <title>Main Outcome Measures</title> <p>The aqueous and vitreous levels of D‐serine and glutamate were measured with reverse‐phase high‐performance liquid chromatography (HPLC); the contents of haemoglobin in the blood and in the vitreous specimens from PDR were measured with spectrophotometry to correct possible introduction of amino acids from PDR haemorrhage.</p> </sec> <sec id="ceo12329-sec-0006" sec-type="section"> <title>Results</title> <p>The concentrations of D‐serine in the aqueous or vitreous humour were significantly higher in patients with PDR compared with control subjects. The vitreous concentrations of D‐serine in PDR were 25.55 ± 0.63 μmol/L compared with control subjects at 22.76 ± 0.36 μmol/L (<italic>P</italic> = 0.002); the levels of D‐serine in the aqueous humour from patients with PDR were 29.08 ± 1.31 μmol/L compared with control subjects at 24.22 ± 0.65 μmol/L (<italic>P</italic> = 0.006). Correction from possible introduction of D‐serine from the vitreous haemorrhage in PDR did not significantly alter the findings.</p> </sec> <sec id="ceo12329-sec-0007" sec-type="section"> <title>Conclusions</title> <p>Increased D‐serine in the aqueous and vitreous humour was found in patients with PDR compared with control subjects.</p> </sec> </abstract>