Inhibitory and anti‐inflammatory effects of the Helicobacter pylori‐derived antimicrobial peptide HPA3NT3 against Propionibacterium acnes in the skin. (9th November 2014)

• Record Type: Journal Article
• Authors: Ryu, S.; Park, Y.; Kim, B.; Cho, S.‐M.; Lee, J.; Lee, H.‐H.; Gurley, C.; Song, K.; Johnson, A.; Armstrong, C.A.; Song, P.I.
• Is Part Of: British journal of dermatology. Volume 171:Number 6(2014:Dec.)
• Page Start: 1358
• Page End: 1367
• Publication Date: 2014-11-09
• Subjects: Dermatology -- Periodicals; Skin -- Diseases -- Periodicals; 616.5
• DOI: 10.1111/bjd.13480
• Languages: English
• ISSNs: 0007-0963

Abstract

<abstract abstract-type="main" id="bjd13480-abs-0001"> <title>Summary</title> <sec id="bjd13480-sec-0001" sec-type="section"> <title>Background</title> <p>An effective treatment strategy for acne vulgaris is the reduction of <italic>Propionibacterium acnes</italic> in the skin. The <italic>Helicobacter pylori</italic>‐derived synthetic antimicrobial peptide HPA3NT3 is a customized α‐helical cationic peptide with antibacterial and anti‐inflammatory activity.</p> </sec> <sec id="bjd13480-sec-0002" sec-type="section"> <title>Objectives</title> <p>To examine the role of HPA3NT3 as a treatment against <italic>P. acnes</italic>‐induced skin inflammation.</p> </sec> <sec id="bjd13480-sec-0003" sec-type="section"> <title>Methods</title> <p>Morphological alteration of individual <italic>P. acnes</italic> cells by HPA3NT3 was visualized by scanning electron microscopy. Modulation by HPA3NT3 of a number of <italic>P. acnes</italic>‐induced innate immune responses was analysed <italic>in vitro</italic> using cultured normal human keratinocytes (HKs), and <italic>in vivo</italic> using the ICR mouse, a well‐established model for <italic>P. acnes</italic>‐induced skin inflammation.</p> </sec> <sec id="bjd13480-sec-0004" sec-type="section"> <title>Results</title> <p>The minimum inhibitory concentration of HPA3NT3 against <italic>P. acnes</italic> was low (0·4 μmol L<sup>−1</sup>). HPA3NT3 showed no cytotoxicity to HK cells at the concentrations used in our <italic>in vitro</italic> and <italic>in vivo</italic> studies. Treatment with HPA3NT3 <italic>in vitro</italic> induced morphological disruptions in <italic>P. acnes</italic> cells suggestive of a bactericidal effect. HPA3NT3 significantly decreased <italic>P. acnes</italic>‐induced interleukin‐8 expression and intracellular calcium mobilization in HK cells by inhibiting <italic>P. acnes</italic>‐activated Toll‐like receptor 2‐mediated nuclear factor‐κB signalling pathways. Intradermal injection of HPA3NT3 <italic>in vivo</italic> effectively decreased viable <italic>P. acnes</italic>, as well as erythema, swelling and inflammatory‐cell infiltration in ICR mouse ears inoculated with <italic>P. acnes</italic>.</p> </sec> <sec id="bjd13480-sec-0005" sec-type="section"> <title>Conclusions</title> <p>Our data suggest that HPA3NT3 has potential as a therapeutic agent for acne vulgaris due to its antimicrobial effects on <italic>P. acnes</italic> and its ability to block <italic>P. acnes</italic>‐induced inflammation.</p> </sec> </abstract>