A new and highly sensitive resonance Rayleigh scattering assay for lysozyme using aptamer–nanogold as a probe. (10th April 2014)

• Record Type: Journal Article
• Authors: Ma, Lu; Zhang, Xinghui; Liang, Aihui; Liu, Qingye; Jiang, Zhiliang
• Is Part Of: Luminescence. Volume 29:Number 8(2014)
• Page Start: 1003
• Page End: 1007
• Publication Date: 2014-04-10
• Subjects: Luminescence -- Periodicals; Bioluminescence -- Periodicals; Chemiluminescence -- Periodicals; Luminescence -- Periodicals; 535.35
• DOI: 10.1002/bio.2650
• Languages: English
• ISSNs: 1522-7235

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>Gold nanoparticles (GN), 10 nm in size, were modified by using lysozyme aptamer (Apt) to obtain a stable Apt–GN probe in pH 8.05 Tris/HCl buffer solutions containing 0.04 mol/L NaCl. Upon addition of lysozyme (LYS), it reacted with the Apt of the probe to form a very stable Apt–LYS complex and to release GNs, which aggregated to form large clusters with a resonance Rayleigh scattering (RRS) peak at 368 nm. The enhanced peak intensity, Δ<italic>I</italic>, was linear to the LYS concentration in the range 0.2–5.2 nmol/L, with a detection limit of 0.05 nmol/L. The influence of foreign substance was tested, and the results showed that this RRS method has high selectivity. This Apt–GN RRS method was applied to the analysis of LYS in a real sample, with satisfactory results. Copyright © 2014 John Wiley &amp; Sons, Ltd.</p> </abstract>