Enhanced production of 2, 3‐butanediol by overexpressing acetolactate synthase and acetoin reductase in Klebsiella pneumoniae. (29th April 2014)

• Record Type: Journal Article
• Authors: Guo, Xue‐Wu; Zhang, Yun‐Hui; Cao, Chun‐Hong; Shen, Tong; Wu, Ming‐Yue; Chen, Ye‐Fu; Zhang, Cui‐Ying; Xiao, Dong‐Guang
• Is Part Of: Biotechnology and applied biochemistry. Volume 61:Number 6(2014)
• Page Start: 707
• Page End: 715
• Publication Date: 2014-04-29
• Subjects: Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.1217
• Languages: English
• ISSNs: 0885-4513

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Mutants with overexpression of α‐acetolactate synthase (ALS), α‐acetolactate decarboxylase, and acetoin reductase (AR), either individually or in combination, were constructed to improve 2, 3‐butanediol (2, 3‐BD) production in <italic>Klebsiella pneumoniae</italic>. The recombinant strains were characterized in terms of the enzyme activity, 2, 3‐BD yield, and expression levels. The recombinant <italic>K. pneumoniae</italic> strain (KG‐rs) that overexpressed both ALS and AR showed an improved 2, 3‐BD yield. When cultured in the media with five different carbon sources (glucose, galactose, fructose, sucrose, and lactose), the mutant exhibited higher 2, 3‐BD productivity and production than the parental strain in all the tested carbon sources except for lactose. The 2, 3‐BD production of KG‐rs in a batch fermentation with glucose as the carbon source was 12% higher than that of the parental strain.</p> </abstract>