Detection of the I1781L mutation in fenoxaprop‐p‐ethyl‐resistant American sloughgrass (Beckmannia syzigachne Steud.), based on the loop‐mediated isothermal amplification method. Issue 1 (15th April 2014)

• Record Type: Journal Article
• Authors: Pan, Lang; Li, Jun; Zhang, Wen‐na; Dong, Liyao
• Is Part Of: Pest management science. Volume 71:Issue 1(2015:Jan.)
• Page Start: 123
• Page End: 130
• Publication Date: 2014-04-15
• Subjects: Pests -- Control -- Periodicals; Pesticides -- Periodicals; 632.9
• DOI: 10.1002/ps.3777
• Languages: English
• ISSNs: 1526-498X

Abstract

<abstract abstract-type="main" id="ps3777-abs-0001"> <title>Abstract</title> <sec id="ps3777-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p id="ps3777-para-0001">The increasing use of fenoxaprop‐<italic>p</italic>‐ethyl has resulted in evolved resistance in American sloughgrass (<italic>Beckmannia syzigachne</italic> Steud.). Target‐site‐based resistance to acetyl‐CoA carboxylase (ACCase) inhibitors in <italic>B. syzigachne</italic> occurs owing to an isoleucine‐to‐leucine substitution at residue 1781 (I1781L) of the ACCase enzyme. A rapid detection method is needed to identify the resistance‐conferring substitution.</p> </sec> <sec id="ps3777-sec-0002" sec-type="section"> <title>RESULTS</title> <p id="ps3777-para-0002">Four populations of <italic>B. syzigachne</italic> that were resistant to fenoxaprop‐<italic>p</italic>‐ethyl and contained the I1781L substitution were identified. Conventional PCR and derived cleaved amplified polymorphic sequence (dCAPS) methods were used to detect the mutation. Additionally, a rapid nucleic acid detection method, loop‐mediated isothermal amplification (LAMP), was successfully developed and used to detect the genetic mutation underlying the I1781L substitution in the <italic>B. syzigachne</italic> ACCase enzyme.</p> </sec> <sec id="ps3777-sec-0003" sec-type="section"> <title>CONCLUSION</title> <p id="ps3777-para-0003">This report is the first to describe the application of a LAMP assay for mutation detection in herbicide‐resistant weeds. The assay does not require specialised equipment: only a standard laboratory bath is needed. This technique could be employed for detecting the I1781L substitution in <italic>B. syzigachne</italic> plants and seeds. © 2014 Society of Chemical Industry</p> </sec> </abstract>