Enhanced polyaromatic hydrocarbon degradation by adapted cultures of actinomycete strains. (10th September 2014)

• Record Type: Journal Article
• Authors: Bourguignon, Natalia; Isaac, Paula; Alvarez, Héctor; Amoroso, María J.; Ferrero, Marcela A.
• Is Part Of: Journal of basic microbiology. Volume 54:issue 12(2014:Dec.)
• Page Start: 1288
• Page End: 1294
• Publication Date: 2014-09-10
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.201400262
• Languages: English
• ISSNs: 0233-111X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jobm201400262-sec-0001" sec-type="section"> <p>Fifteen actinomycete strains were evaluated for their potential use in removal of polycyclic aromatic hydrocarbons (PAH). Their capability to degrade of naphthalene, phenanthrene, and pyrene was tested in minimal medium (MM) and MM with glucose as another substrate. Degradation of naphthalene in MM was observed in all isolates at different rates, reaching maximum values near to 76% in some strains of <italic>Streptomyces</italic>, <italic>Rhodococcus</italic> sp. 016 and <italic>Amycolatopsis tucumanensis</italic> DSM 45259. Maximum values of degradation of phenanthrene in MM occurred in cultures of <italic>A. tucumanensis</italic> DSM 45259 (36.2%) and <italic>Streptomyces</italic> sp. A12 (20%), while the degradation of pyrene in MM was poor and only significant with <italic>Streptomyces</italic> sp. A12 (4.3%). Because of the poor performance when growing on phenanthrene and pyrene alone, <italic>Rhodococcus</italic> sp. 20, <italic>Rhodococcus</italic> sp. 016, <italic>A. tucumanensis</italic> DSM 45259, <italic>Streptomyces</italic> sp. A2, and <italic>Streptomyces</italic> sp. A12 were challenged to an adaptation schedule of successive cultures on a fresh solid medium supplemented with PAHs, decreasing concentration of glucose in each step. As a result, an enhanced degradation of PAHs by adapted strains was observed in the presence of glucose as co‐substrate, without degradation of phenanthrene and pyrene in MM while an increase to up to 50% of degradation was seen with these strains in glucose amended media. An internal fragment of the <italic>catA</italic> gene, which codes for catechol 1, 2‐dioxygenase, was amplified from both <italic>Rhodococcus</italic> strains, showing the potential for degradation of aromatic compounds via salycilate. These results allow us to propose the usefulness of these actinomycete strains for PAH bioremediation in the environment.</p> </sec> </abstract>