Molecularly Imprinted Polymer Waveguides for Direct Optical Detection of Low‐Molecular‐Weight Analytes. Issue 23 (25th September 2014)

• Record Type: Journal Article
• Authors: Sharma, Nityanand; Petri, Christian; Jonas, Ulrich; Bach, Monika; Tovar, Günter; Mrkvová, Kateřina; Vala, Milan; Homola, Jiří; Knoll, Wolfgang; Dostálek, Jakub
• Is Part Of: Macromolecular chemistry and physics. Volume 215:Issue 23(2014:Dec.)
• Page Start: 2295
• Page End: 2304
• Publication Date: 2014-09-25
• Subjects: Polymers -- Periodicals; Polymerization -- Periodicals; Synthetic products -- Periodicals; Macromolecules -- Periodicals; 547.7
• DOI: 10.1002/macp.201400260
• Languages: English
• ISSNs: 1022-1352

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>New composite layer architecture of 3D hydrogel polymer network that is loaded with mole­cularly imprinted polymer nanoparticles (nanoMIP) is reported for direct optical detection of low‐molecular‐weight compounds. This composite layer is attached to the metallic surface of a surface plasmon resonance (SPR) sensor in order to simultaneously serve as an optical waveguide and large capacity binding‐matrix for imprinted target analyte. Optical waveguide spectroscopy (OWS) is used as a label‐free readout method allowing direct measurement of refractive index changes that are associated with molecular binding events inside the matrix. This approach is implemented by using a photo‐crosslinkable poly(<italic>N</italic>‐isopropylacrylamide)‐based hydrogel and poly[(ethylene glycol dimethylacrylate)‐(methacrylic acid)] nanoparticles that are imprinted with <sc>l</sc>‐Boc‐phenylalanine‐anilide (<sc>l</sc>‐BFA, molecular weight 353 g mol<sup>−1</sup>). Titration experiments with the specific target and other structurally similar reference compounds show good specificity and limit of detection for target <sc>l</sc>‐BFA as low as 2 × 10<sup>−6</sup><sc>m</sc>. <boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgh2hn0c8t7" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>