Determination of drugs of abuse in bovine dentin using liquid chromatography–electrospray ionization tandem mass spectrometry. (December 2014)

• Record Type: Journal Article
• Authors: Spinner, J.; Klima, M.; Kempf, J.; Huppertz, L. M.; Auwärter, V.; Altenburger, M. J.; Neukamm, M. A.
• Is Part Of: Journal of mass spectrometry. Volume 49:Number 12(2014:Dec.)
• Page Start: 1306
• Page End: 1313
• Publication Date: 2014-12
• Subjects: Mass spectrometry -- Periodicals; 543.65
• DOI: 10.1002/jms.3464
• Languages: English
• ISSNs: 1076-5174

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Drugs deposited in human teeth are well preserved; the spectrum of toxicological investigations may therefore be supplemented by an analysis method for drugs in teeth. A liquid chromatography–electrospray ionization tandem mass spectrometry assay for the detection and quantification of basic drugs of abuse in bovine dentin samples was developed and validated. The drugs and metabolites amphetamine, methamphetamine, methylenedioxymethylamphetamine, methylenedioxyethylamphetamine, codeine, morphine, cocaine and benzoylecgonine were extracted from 50 mg ground dentin powder by ultrasonication for 60 min in methanol 3 times. The extracts were analyzed on a triple‐quadrupole mass‐spectrometer in multiple reaction monitoring mode. The method was validated and proved to be accurate, precise, selective, specific and stable with good linearity within the calibration range and a lower limit of quantification of 10 to 20 pg/mg. To artificially load bovine dentin samples with drugs, the natural process of de‐ and remineralization in the oral cavity was mimicked by a pH‐cycling experiment. The artificially drug‐loaded dentin samples showed drug concentrations of 20 to 80 pg/mg. The method can be applied in further <italic>in vitro</italic> experiments as well as in post‐mortem cases, especially where limited sample tissue is available. Copyright © 2014 John Wiley &amp; Sons, Ltd.</p> </abstract>