Sensitive Phenol Detection Using Tyrosinase‐Based Phenol Oxidation Combined with Redox Cycling of Catechol. Issue 12 (17th November 2014)

• Record Type: Journal Article
• Authors: Noh, Soodong; Yang, Haesik
• Is Part Of: Electroanalysis. Volume 26:Issue 12(2014:Dec.)
• Page Start: 2727
• Page End: 2731
• Publication Date: 2014-11-17
• Subjects: Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201400383
• Languages: English
• ISSNs: 1040-0397

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>This paper reports sensitive phenol detection using (i) tyrosinase (Tyr)‐based oxidation of phenol to catechol, combined with (ii) electrochemical‐chemical‐chemical (ECC) redox cycling involving Ru(NH₃)₆³⁺, catechol, and tris(2‐carboxyethyl)phosphine (TCEP). Phenol is converted into catechol by Tyr in the presence of dissolved O₂. Catechol then reacts with Ru(NH₃)₆³⁺, generating <italic>o</italic>‐benzoquinone and Ru(NH₃)₆²⁺. <italic>o</italic>‐Benzoquinone is reduced back to catechol by TCEP, and Ru(NH₃)₆²⁺ is accumulated over the course of the incubation. When Ru(NH₃)₆²⁺ is electrochemically oxidized to Ru(NH₃)₆³⁺, ECC redox cycling occurs. For simple phenol detection, bare ITO electrodes are used without modifying the electrodes with Tyr. The detection limit for phenol in tap water using Tyr‐based oxidation combined with ECC redox cycling is ca. 10⁻⁹ M, while that using only Tyr‐based oxidation is ca. 10⁻⁷ M.</p> </abstract>