Control of Cellular Function by Reversible Photoregulation of Translation. Issue 18 (28th October 2014)

• Record Type: Journal Article
• Authors: Ogasawara, Shinzi
• Is Part Of: Chembiochem. Volume 15:Issue 18(2014)
• Page Start: 2652
• Page End: 2655
• Publication Date: 2014-10-28
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201402495
• Languages: English
• ISSNs: 1439-4227

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>The use of light as an external stimulus offers the potential for spatiotemporal control and is thus ideal for controlling gene expression in living cells. In commonly used caging systems, once the caging compound is removed, protein expression cannot be stopped, due to the irreversibility of the uncaging reaction. We have developed a reversible method for regulating protein expression with the aid of a photoresponsive cap that can control the translation of mRNA in a reversible manner through triggering of <italic>cis</italic>–<italic>trans</italic> photoisomerization of the cap. In its <italic>trans</italic> form, the photoresponsive cap completely inhibited translation, whereas the <italic>cis</italic> form yielded protein (12.7 times more translated protein than in the <italic>trans</italic> form). Moreover, we succeeded in controlling the levels, timing and duration of protein expression in living mammalian cells. Additionally, neuronal differentiation of PC12 cells was photoinduced by controlling constitutively active H‐Ras 61L protein expression.</p> </abstract>