Maximizing recombinant human serum albumin production in a MutsPichia pastoris strain. (16th September 2014)

• Record Type: Journal Article
• Authors: Mallem, Muralidhar; Warburton, Shannon; Li, Fang; Shandil, Ishaan; Nylen, Adam; Kim, Sehoon; Jiang, Youwei; Meehl, Michael; d'Anjou, Marc; Stadheim, Terrance A.; Choi, Byung‐Kwon
• Is Part Of: Biotechnology progress. Volume 30:Number 6(2014)
• Page Start: 1488
• Page End: 1496
• Publication Date: 2014-09-16
• Subjects: Biotechnology -- Periodicals; Food industry and trade -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/btpr.1990
• Languages: English
• ISSNs: 8756-7938

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Human serum albumin (HSA) is a cysteine rich molecule that is most abundant in human blood plasma. To remain viable in the market due to lower marketing costs for HSA, it is important to produce a large quantity in an economical manner by recombinant technology. The objective of this study was to maximize recombinant HSA (rHSA) production using a Mut^s Pichia pastoris strain by fermentation process optimization. We evaluated the impact of process parameters on the production of rHSA, including induction cell density (wet cell weight, g/L) and the control of specific growth rate at induction. In this study, we demonstrated that induction cell density is a critical factor for high level production of rHSA under controlled specific growth rate. We observed higher specific productivities at higher induction cell densities (285 g/L) and at lower specific growth rates (0.0022–0.0024/h) during methanol induction phase, and achieved the broth titer of rHSA up to 10 g/L. The temperature shift from 24 to 28^oC was effective to control the specific growth rate at low level (≤0.0024/h) during methanol induction phase while maintaining high specific productivity [0.0908 mg_rHSA/(g_wcw h)]. © 2014 American Institute of Chemical Engineers <italic>Biotechnol. Prog</italic>., 30:1488–1496, 2014</p> </abstract>