High prevalence of pfmdr1 N86Y and Y184F mutations in Plasmodium falciparum isolates from Bioko island, Equatorial Guinea. (October 2014)

• Record Type: Journal Article
• Authors: Li, Jian; Chen, Jiangtao; Xie, Dongde; Monte-Nguba, Santiago-m; Eyi, Juan Urbano Monsuy; Matesa, Rocio Apicante; Obono, Maximo Miko Ondo; Ehapo, Carlos Sala; Yang, Liye; Lu, Danjie; Yang, Hui; Yang, Hui-Tian; Lin, Min
• Is Part Of: Pathogens and global health. Volume 108:Number 7(2014)
• Page Start: 339
• Page End: 343
• Publication Date: 2014-10
• Subjects: Communicable diseases -- Periodicals; Public health -- International cooperation -- Periodicals; World health -- Periodicals; 362.1969
• DOI: 10.1179/2047773214Y.0000000158
• Languages: English
• ISSNs: 2047-7724

Abstract

<abstract> <title> <x content-type="archive" xml:space="preserve">Abstract</x> </title> <sec> <title>Objective:</title> <p>Drug resistance against <italic>Plasmodium falciparum</italic> has been recognized as the crucial obstacle to curbing mortality and morbidity from malaria. To investigate the distribution and pattern of multidrug resistance 1 (pfmdr1) gene polymorphisms in <italic>P. falciparum</italic>, isolates collected from the malaria high-endemic Bioko Island, Equatorial Guinea.</p> </sec> <sec> <title>Methods:</title> <p>Blood samples were collected from 217 patients with <italic>P. falciparum</italic> malaria during rainy season in 2012 on Bioko Island. These samples were extracted using Chelex to obtain parasite DNA. Nest-polymerase chain reaction (PCR) and sequencing were employed to detect mutations (N86Y, E130K, Y184F, S1034C, N1042D, V1109I, and D1246Y) and haplotypes in pfmdr1 gene.</p> </sec> <sec> <title>Results:</title> <p>A total of 151 samples were successfully detected for pfmdr1 mutations from the 217 patients. Pfmdr1 mutations were found in 91·39% (138/151) <italic>P. falciparum</italic> isolates. However, no mutation at 130 and 1109 was identified from these samples. Four haplotypes coding 86, 184, 1034, 1 042, and 1 246 were found including NYSND, YYSND, NFSND, and YFSND, which accounted for 8·61% (13/151), 2·65% (4/151), 29·80% (45/151), and 58·94% (89/151), respectively.</p> </sec> <sec> <title>Conclusions:</title> <p>Our results exhibited hypersensitivity to lumefantrine (LU) and mefloquine (MQ) and resistance to chloroquine (CQ) and amodiaquine (AQ) in <italic>P. falciparum</italic> isolates from Bioko Island. This information will be useful for anti-malarial drug policy in Equatorial Guinea.</p> </sec> </abstract>